Binding of [14C]azaserine to DNA and protein in the rat and hamster

Joanne Zurlo; Charles I. Coon; Daniel S. Longnecker; Thomas J. Curphey

doi:10.1016/0304-3835(82)90092-1

Binding of [¹⁴C]azaserine to DNA and protein in the rat and hamster

Joanne Zurlo, Charles I. Coon, Daniel S. Longnecker, Thomas J. Curphey

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The binding of [¹⁴C]azaserine or its metabolites to DNA and protein in the organs of rats and hamsters was determined at various times after treatment with [¹⁴C]azaserine. The specific activity of ¹⁴C labelling of DNA and protein was determined. Rat liver DNA and protein were most extensively labelled at 90 min post-injection, but by 24 h the specific activity decreased to the levels found in pancreas and kidney. Thymus contained negligible amounts of radioactivity at all time-points. DNA and protein from hamster pancreas contained more label than did DNA and protein from rat pancreas. The results suggest that factors other than DNA binding play a role in determining the species and organ specificity of azaserine.

Original language	English (US)
Pages (from-to)	65-70
Number of pages	6
Journal	Cancer Letters
Volume	16
Issue number	1
DOIs	https://doi.org/10.1016/0304-3835(82)90092-1
State	Published - 1982
Externally published	Yes

ASJC Scopus subject areas

Oncology
Cancer Research

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10.1016/0304-3835(82)90092-1

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@article{7034dee680cb4d3196f8363f0cb3bf79,

title = "Binding of [14C]azaserine to DNA and protein in the rat and hamster",

abstract = "The binding of [14C]azaserine or its metabolites to DNA and protein in the organs of rats and hamsters was determined at various times after treatment with [14C]azaserine. The specific activity of 14C labelling of DNA and protein was determined. Rat liver DNA and protein were most extensively labelled at 90 min post-injection, but by 24 h the specific activity decreased to the levels found in pancreas and kidney. Thymus contained negligible amounts of radioactivity at all time-points. DNA and protein from hamster pancreas contained more label than did DNA and protein from rat pancreas. The results suggest that factors other than DNA binding play a role in determining the species and organ specificity of azaserine.",

author = "Joanne Zurlo and Coon, {Charles I.} and Longnecker, {Daniel S.} and Curphey, {Thomas J.}",

note = "Funding Information: The authors wish to thank Dr. James D. Yager, Jr. for his critical review of this manuscript and Maureen Freitag for assistance in its preparation. This work was supported by NIH grant CA 17843 and NIH Postdoctoral Fellowship F32-CA 06855 (Dr. Zurlo).",

year = "1982",

doi = "10.1016/0304-3835(82)90092-1",

language = "English (US)",

volume = "16",

pages = "65--70",

journal = "Cancer Letters",

issn = "0304-3835",

publisher = "Elsevier Ireland Ltd",

number = "1",

}

TY - JOUR

T1 - Binding of [14C]azaserine to DNA and protein in the rat and hamster

AU - Zurlo, Joanne

AU - Coon, Charles I.

AU - Longnecker, Daniel S.

AU - Curphey, Thomas J.

N1 - Funding Information: The authors wish to thank Dr. James D. Yager, Jr. for his critical review of this manuscript and Maureen Freitag for assistance in its preparation. This work was supported by NIH grant CA 17843 and NIH Postdoctoral Fellowship F32-CA 06855 (Dr. Zurlo).

PY - 1982

Y1 - 1982

N2 - The binding of [14C]azaserine or its metabolites to DNA and protein in the organs of rats and hamsters was determined at various times after treatment with [14C]azaserine. The specific activity of 14C labelling of DNA and protein was determined. Rat liver DNA and protein were most extensively labelled at 90 min post-injection, but by 24 h the specific activity decreased to the levels found in pancreas and kidney. Thymus contained negligible amounts of radioactivity at all time-points. DNA and protein from hamster pancreas contained more label than did DNA and protein from rat pancreas. The results suggest that factors other than DNA binding play a role in determining the species and organ specificity of azaserine.

AB - The binding of [14C]azaserine or its metabolites to DNA and protein in the organs of rats and hamsters was determined at various times after treatment with [14C]azaserine. The specific activity of 14C labelling of DNA and protein was determined. Rat liver DNA and protein were most extensively labelled at 90 min post-injection, but by 24 h the specific activity decreased to the levels found in pancreas and kidney. Thymus contained negligible amounts of radioactivity at all time-points. DNA and protein from hamster pancreas contained more label than did DNA and protein from rat pancreas. The results suggest that factors other than DNA binding play a role in determining the species and organ specificity of azaserine.

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JO - Cancer Letters

JF - Cancer Letters

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Binding of [¹⁴C]azaserine to DNA and protein in the rat and hamster

Abstract

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