Binding of [14C]azaserine to DNA and protein in the rat and hamster

Joanne Zurlo, Charles I. Coon, Daniel S. Longnecker, Thomas J. Curphey

Research output: Contribution to journalArticle

Abstract

The binding of [14C]azaserine or its metabolites to DNA and protein in the organs of rats and hamsters was determined at various times after treatment with [14C]azaserine. The specific activity of 14C labelling of DNA and protein was determined. Rat liver DNA and protein were most extensively labelled at 90 min post-injection, but by 24 h the specific activity decreased to the levels found in pancreas and kidney. Thymus contained negligible amounts of radioactivity at all time-points. DNA and protein from hamster pancreas contained more label than did DNA and protein from rat pancreas. The results suggest that factors other than DNA binding play a role in determining the species and organ specificity of azaserine.

Original languageEnglish (US)
Pages (from-to)65-70
Number of pages6
JournalCancer Letters
Volume16
Issue number1
DOIs
StatePublished - Jan 1 1982

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Zurlo, J., Coon, C. I., Longnecker, D. S., & Curphey, T. J. (1982). Binding of [14C]azaserine to DNA and protein in the rat and hamster. Cancer Letters, 16(1), 65-70. https://doi.org/10.1016/0304-3835(82)90092-1