Binding of [14C]azaserine to DNA and protein in the rat and hamster

Joanne Zurlo, Charles I. Coon, Daniel S. Longnecker, Thomas J. Curphey

Research output: Contribution to journalArticlepeer-review

Abstract

The binding of [14C]azaserine or its metabolites to DNA and protein in the organs of rats and hamsters was determined at various times after treatment with [14C]azaserine. The specific activity of 14C labelling of DNA and protein was determined. Rat liver DNA and protein were most extensively labelled at 90 min post-injection, but by 24 h the specific activity decreased to the levels found in pancreas and kidney. Thymus contained negligible amounts of radioactivity at all time-points. DNA and protein from hamster pancreas contained more label than did DNA and protein from rat pancreas. The results suggest that factors other than DNA binding play a role in determining the species and organ specificity of azaserine.

Original languageEnglish (US)
Pages (from-to)65-70
Number of pages6
JournalCancer Letters
Volume16
Issue number1
DOIs
StatePublished - 1982

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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