Binding of preformed xenoantibodies to porcine bioprosthetic valves

Juan A. Sanchez, Charles C. Marboe, Joseph S. Auteri, Valluvan Jeevanandum, Niloo M. Edwards, Carole L. Berger, Eric A. Rose

Research output: Contribution to journalArticle

Abstract

We have investigated whether preformed antibodies against xenoantigens bind to cellular elements remaining on porcine bioprosthetic valves after various methods of preservation. Fresh porcine valves treated with either acetone, 4% formaldehyde, or 0.625% glutaraldehyde, as well as an unfixed valve, were incubated with antiserum against porcine xenoantigens. This serum was prepared using the affinity purification method with porcine lymphocytes as the target. The valves were stained with secondary fluorescein-conjugated antibody against immunoglobulin M or immunoglobulin G and examined under fluorescent microscopy. Intense binding of immunoglobulin M to the endocardium was observed in the unfixed valve as well as in valves fixed in acetone and formaldehyde. Glutaraldehyde fixation eliminated binding of antibody. Binding was not noted within the connective tissue. No binding of antiimmunoglobulin G was noted on the endocardium of any of the sections. Examination of three glutaraldehyde-treated porcine valves explanted from the aortic position after 10 years in situ showed no immunoglobulin deposition. These results demonstrate the elimination of antigenicity to preformed antibodies in the endocardium and connective tissue of glutaraldehyde-preserved porcine valves. The findings may, in part, explain the poor performance of formaldehyde-preserved bioprosthetic xenograft valves in the past and support the use of glutaraldehyde as a preferred agent for preservation of bioprosthetic endovascular materials.

Original languageEnglish (US)
Pages (from-to)30-33
Number of pages4
JournalThe Annals of thoracic surgery
Volume51
Issue number1
DOIs
StatePublished - Jan 1991
Externally publishedYes

ASJC Scopus subject areas

  • Surgery
  • Pulmonary and Respiratory Medicine
  • Cardiology and Cardiovascular Medicine

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  • Cite this

    Sanchez, J. A., Marboe, C. C., Auteri, J. S., Jeevanandum, V., Edwards, N. M., Berger, C. L., & Rose, E. A. (1991). Binding of preformed xenoantibodies to porcine bioprosthetic valves. The Annals of thoracic surgery, 51(1), 30-33. https://doi.org/10.1016/0003-4975(91)90441-R