Binding of nerve growth factor receptor in sympathetic ganglia

S. P. Banerjee, Solomon H Snyder, P. Cuatrecasas, L. A. Greene

Research output: Contribution to journalArticle

Abstract

125I Labeled nerve growth factor binds to membrane preparations from superior cervical ganglia of rabbit in a specific fashion indicative of an interaction with the physiological receptor. Of various tissues examined, binding was observed only in the superior cervical ganglia, a presumed target organ of nerve growth factor. Binding of labeled factor is displaced by nonradioactive factor but not by several other peptide hormones. Binding to ganglia of preparations of nerve growth factor treated with N bromosuccinimide closely parallels their biological activity. Binding of nerve growth factor receptor is saturable with an affinity constant of about 0.2 nM, similar to the reported plasma levels of the factor. Nerve growth factor does not elicit insulin like biological effects in isolated adipocytes, and it does not modify the binding of [125I]insulin or [125I]epidermal growth factor to fat cells or liver membranes.

Original languageEnglish (US)
Pages (from-to)2519-2523
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume70
Issue number9
StatePublished - 1973

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Nerve Growth Factor Receptor
Sympathetic Ganglia
Nerve Growth Factor
Superior Cervical Ganglion
Adipocytes
Bromosuccinimide
Insulin
Membranes
Peptide Hormones
Epidermal Growth Factor
Ganglia
Rabbits
Liver

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Binding of nerve growth factor receptor in sympathetic ganglia. / Banerjee, S. P.; Snyder, Solomon H; Cuatrecasas, P.; Greene, L. A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 70, No. 9, 1973, p. 2519-2523.

Research output: Contribution to journalArticle

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