Binding characteristics of S fimbriated Escherichia coli to isolated brain microvascular endothelial cells

Monique Stins, Nemani V. Prasadarao, Luminita Ibric, Carol A. Wass, Peter Luckett, Kwang Sik Kim

Research output: Contribution to journalArticle

Abstract

To assess the role of S fimbriae in the pathogenesis of Escherichia coli meningitis, transformants of E. coli strains with or without S fimbriae plasmid were compared for their binding to microvessel endothelial cells isolated from bovine brain cortices (BMEC). The BMEC's displayed a cobblestone appearance, were positive for factor VIII, carbonic anhydrase IV, took up fluorescent-labeled acetylated low density lipoprotein, and exhibited gamma glutamyl transpeptidase activity. Binding of S fimbriated E. coli to BMEC was approximately threefold greater than nonfimbriated E. coli Similarly S fimbriated E. coli bound to human brain endothelial cells approximately threefold greater than nonfimbriated E. coli Binding was reduced approximately 60% by isolated S fimbriae and about 80% by anti-S adhesin antibody. Mutating the S adhesin gene resulted in a complete loss of the binding, whereas mutagenesis of the major S fimbriae subunit gene sfaA did not significantly affect binding. Pretreatment of BMEC with neuraminidase or prior incubation of S fimbriated E. coli with NeuAcα2,3-sialyl lactose completely abolished binding. These findings indicate that S fimbriated E. coli bind to NeuAcα2,3-galactose containing glycoproteins on brain endothelial cells via a lectin-like activity of SfaS adhesin. This might be an important early step in the penetration of bacteria across the blood- brain barrier in the development of E. coli meningitis.

Original languageEnglish (US)
Pages (from-to)1228-1236
Number of pages9
JournalAmerican Journal of Pathology
Volume145
Issue number5
StatePublished - Nov 1994
Externally publishedYes

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Endothelial Cells
Escherichia coli
Brain
Escherichia coli Meningitis
Carbonic Anhydrase IV
gamma-Glutamyltransferase
Factor VIII
Neuraminidase
Microvessels
Blood-Brain Barrier
Galactose
Lectins
Mutagenesis
Genes
Glycoproteins
Plasmids
Bacteria
Antibodies

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Binding characteristics of S fimbriated Escherichia coli to isolated brain microvascular endothelial cells. / Stins, Monique; Prasadarao, Nemani V.; Ibric, Luminita; Wass, Carol A.; Luckett, Peter; Kim, Kwang Sik.

In: American Journal of Pathology, Vol. 145, No. 5, 11.1994, p. 1228-1236.

Research output: Contribution to journalArticle

Stins, Monique ; Prasadarao, Nemani V. ; Ibric, Luminita ; Wass, Carol A. ; Luckett, Peter ; Kim, Kwang Sik. / Binding characteristics of S fimbriated Escherichia coli to isolated brain microvascular endothelial cells. In: American Journal of Pathology. 1994 ; Vol. 145, No. 5. pp. 1228-1236.
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