Bidirectional Transepithelial IgG Transport by a Strongly Polarized Basolateral Membrane Fcγ-Receptor

Steven M. Claypool, Bonny L. Dickinson, Jessica S. Wagner, Finn Eirik Johansen, Nanda Venu, Jason A. Borawski, Wayne I. Lencer, Richard S. Blumberg

Research output: Contribution to journalArticlepeer-review

Abstract

The human MHC class I-related neonatal Fc receptor, hFcRn, mediates bidirectional transport of IgG across mucosal barriers. Here, we find that at steady state hFcRn distributes predominantly to an apical intracellular compartment and almost exclusively to the basolateral cell surface of polarized epithelial cells. It moves only transiently to the apical membrane. Ligand binding does not redistribute the steady state location of the receptor. Removal of the cytoplasmic tail that contains di-leucine and tryptophan-based endocytosis motifs or incubation at low temperature (18°C) redistributes the receptor apically. The rates of endocytosis of the full-length hFcRn from the apical or basolateral membrane domains, however, are equal. Thus, the strong cell surface polarity displayed by hFcRn results from dominant basolateral sorting by motifs in the cytoplasmic tail that nonetheless allows for a cycle of bidirectional transcytosis.

Original languageEnglish (US)
Pages (from-to)1746-1759
Number of pages14
JournalMolecular biology of the cell
Volume15
Issue number4
DOIs
StatePublished - Apr 2004
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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