Biallelic inactivation of the RIZ1 gene in human gastric cancer

Yutaka Tokumaru; Shuji Nomoto; Carmen Jerónimo; Rui Henrique; Susan Harden; Barry Trink; David Sidransky

doi:10.1038/sj.onc.1206403

Biallelic inactivation of the RIZ1 gene in human gastric cancer

Yutaka Tokumaru, Shuji Nomoto, Carmen Jerónimo, Rui Henrique, Susan Harden, Barry Trink, David Sidransky

School of Medicine

Research output: Contribution to journal › Article › peer-review

53 Scopus citations

Abstract

The distal short arm of chromosome 1 is commonly deleted in a variety of human neoplasms including gastrointestinal cancer. Genetic alterations of the retinoblastoma protein-interacting zing-finger gene (RIZ)1 including loss of heterozygosity (LOH) at 1p36, frameshift mutations, and promoter hypermethylation were reported previously in several cancers. In this study, we evaluated RIZ1 in 30 primary gastric cancers and found frameshift mutations in two cases (6.7%). Moreover, using real-time quantitative methylation-specific PCR, methylation of the RIZ1 promoter was detected in 11 (37%) cases. In all 11 cases with methylation, inactivation of the second allele occurred through frameshift mutation, LOH or promoter methylation. Our results suggest that RIZ1 is a specific target of inactivation in human gastric cancer.

Original language	English (US)
Pages (from-to)	6954-6958
Number of pages	5
Journal	Oncogene
Volume	22
Issue number	44
DOIs	https://doi.org/10.1038/sj.onc.1206403
State	Published - Oct 9 2003

Keywords

Gastric cancer tumor suppressor gene
Gene silencing
Hypermethylation
RIZ1

ASJC Scopus subject areas

Molecular Biology
Genetics
Cancer Research

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10.1038/sj.onc.1206403

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title = "Biallelic inactivation of the RIZ1 gene in human gastric cancer",

abstract = "The distal short arm of chromosome 1 is commonly deleted in a variety of human neoplasms including gastrointestinal cancer. Genetic alterations of the retinoblastoma protein-interacting zing-finger gene (RIZ)1 including loss of heterozygosity (LOH) at 1p36, frameshift mutations, and promoter hypermethylation were reported previously in several cancers. In this study, we evaluated RIZ1 in 30 primary gastric cancers and found frameshift mutations in two cases (6.7%). Moreover, using real-time quantitative methylation-specific PCR, methylation of the RIZ1 promoter was detected in 11 (37%) cases. In all 11 cases with methylation, inactivation of the second allele occurred through frameshift mutation, LOH or promoter methylation. Our results suggest that RIZ1 is a specific target of inactivation in human gastric cancer.",

keywords = "Gastric cancer tumor suppressor gene, Gene silencing, Hypermethylation, RIZ1",

author = "Yutaka Tokumaru and Shuji Nomoto and Carmen Jer{\'o}nimo and Rui Henrique and Susan Harden and Barry Trink and David Sidransky",

note = "Funding Information: We thank Dr Loio Santos for the enrollment of patients in this study. This paper is supported by the NCI/EDRN Grant (1 U01 CA84986) entitled, {\textquoteleft}Integrated Development of Novel Molecular Markers – The Early Detection Research Network.{\textquoteright}",

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AU - Tokumaru, Yutaka

AU - Nomoto, Shuji

AU - Jerónimo, Carmen

AU - Henrique, Rui

AU - Harden, Susan

AU - Trink, Barry

AU - Sidransky, David

N1 - Funding Information: We thank Dr Loio Santos for the enrollment of patients in this study. This paper is supported by the NCI/EDRN Grant (1 U01 CA84986) entitled, ‘Integrated Development of Novel Molecular Markers – The Early Detection Research Network.’

PY - 2003/10/9

Y1 - 2003/10/9

N2 - The distal short arm of chromosome 1 is commonly deleted in a variety of human neoplasms including gastrointestinal cancer. Genetic alterations of the retinoblastoma protein-interacting zing-finger gene (RIZ)1 including loss of heterozygosity (LOH) at 1p36, frameshift mutations, and promoter hypermethylation were reported previously in several cancers. In this study, we evaluated RIZ1 in 30 primary gastric cancers and found frameshift mutations in two cases (6.7%). Moreover, using real-time quantitative methylation-specific PCR, methylation of the RIZ1 promoter was detected in 11 (37%) cases. In all 11 cases with methylation, inactivation of the second allele occurred through frameshift mutation, LOH or promoter methylation. Our results suggest that RIZ1 is a specific target of inactivation in human gastric cancer.

AB - The distal short arm of chromosome 1 is commonly deleted in a variety of human neoplasms including gastrointestinal cancer. Genetic alterations of the retinoblastoma protein-interacting zing-finger gene (RIZ)1 including loss of heterozygosity (LOH) at 1p36, frameshift mutations, and promoter hypermethylation were reported previously in several cancers. In this study, we evaluated RIZ1 in 30 primary gastric cancers and found frameshift mutations in two cases (6.7%). Moreover, using real-time quantitative methylation-specific PCR, methylation of the RIZ1 promoter was detected in 11 (37%) cases. In all 11 cases with methylation, inactivation of the second allele occurred through frameshift mutation, LOH or promoter methylation. Our results suggest that RIZ1 is a specific target of inactivation in human gastric cancer.

KW - Gastric cancer tumor suppressor gene

KW - Gene silencing

KW - Hypermethylation

KW - RIZ1

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Biallelic inactivation of the RIZ1 gene in human gastric cancer

Abstract

Keywords

ASJC Scopus subject areas

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