beta1-integrin mediates myelin-associated glycoprotein signaling in neuronal growth cones.

Eyleen L K Goh, Ju Kim Young, Kenichiro Kuwako, Marc Tessier-Lavigne, Zhigang He, John W. Griffin, Guo Li Ming

Research output: Contribution to journalArticle

Abstract

Several myelin-associated factors that inhibit axon growth of mature neurons, including Nogo66, myelin-associated glycoprotein (MAG) and oligodendrocyte myelin glycoprotein (OMgp), can associate with a common GPI-linked protein Nogo-66 receptor (NgR). Accumulating evidence suggests that myelin inhibitors also signal through unknown NgR-independent mechanisms. Here we show that MAG, a RGD tri-peptide containing protein, forms a complex with β1-integrin to mediate axonal growth cone turning responses of several neuronal types. Mutations that alter the RGD motif in MAG or inhibition of β1-integrin function, but not removal of NgRs, abolish these MAG-dependent events. In contrast, OMgp-induced repulsion is not affected by inhibition of b1-integrin function. We further show that MAG stimulates tyrosine phosphorylation of focal adhesion kinase (FAK), which in turn is required for MAG-induced growth cone turning. These studies identify β1-integrin as a specific mediator for MAG in growth cone turning responses, acting through FAK activation.

Original languageEnglish (US)
Pages (from-to)10
Number of pages1
JournalMolecular Brain
Volume1
StatePublished - 2008
Externally publishedYes

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CD29 Antigens
Myelin-Associated Glycoprotein
Growth Cones
Integrins
Oligodendrocyte-Myelin Glycoprotein
Focal Adhesion Protein-Tyrosine Kinases
Myelin Sheath
GPI-Linked Proteins
Axons
Tyrosine
Phosphorylation
Neurons
Mutation

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Molecular Biology

Cite this

Goh, E. L. K., Young, J. K., Kuwako, K., Tessier-Lavigne, M., He, Z., Griffin, J. W., & Ming, G. L. (2008). beta1-integrin mediates myelin-associated glycoprotein signaling in neuronal growth cones. Molecular Brain, 1, 10.

beta1-integrin mediates myelin-associated glycoprotein signaling in neuronal growth cones. / Goh, Eyleen L K; Young, Ju Kim; Kuwako, Kenichiro; Tessier-Lavigne, Marc; He, Zhigang; Griffin, John W.; Ming, Guo Li.

In: Molecular Brain, Vol. 1, 2008, p. 10.

Research output: Contribution to journalArticle

Goh, ELK, Young, JK, Kuwako, K, Tessier-Lavigne, M, He, Z, Griffin, JW & Ming, GL 2008, 'beta1-integrin mediates myelin-associated glycoprotein signaling in neuronal growth cones.', Molecular Brain, vol. 1, pp. 10.
Goh ELK, Young JK, Kuwako K, Tessier-Lavigne M, He Z, Griffin JW et al. beta1-integrin mediates myelin-associated glycoprotein signaling in neuronal growth cones. Molecular Brain. 2008;1:10.
Goh, Eyleen L K ; Young, Ju Kim ; Kuwako, Kenichiro ; Tessier-Lavigne, Marc ; He, Zhigang ; Griffin, John W. ; Ming, Guo Li. / beta1-integrin mediates myelin-associated glycoprotein signaling in neuronal growth cones. In: Molecular Brain. 2008 ; Vol. 1. pp. 10.
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