Beta structure motifs of islet amyloid polypeptides identified through surface-mediated assemblies

Xiaobo Mao, Chen Xuan Wang, Xing Kui Wu, Xiao Jing Ma, Lei Liu, Lan Zhang, Lin Niu, Yuan Yuan Guo, Deng Hua Li, Yan Lian Yang, Chen Wang

Research output: Contribution to journalArticle

Abstract

We report here the identification of the key sites for the beta structure motifs of the islet amyloid polypeptide (IAPP) analogs by using scanning tunneling microscopy (STM). Duplex folding structures in human IAPP 8-37 (hIAPP 8-37) assembly were observed featuring a hairpin structure. The multiplicity in rIAPP assembly structures indicates the polydispersity of the rat IAPP 8-37 (rIAPP 8-37) beta-like motifs. The bimodal length distribution of beta structure motifs for rIAPP 8-37 R18H indicates the multiple beta segments linked by turns. The IAPP 8-37 analogs share common structure motifs of IAPP 8-17 and IAPP 26-37 with the most probable key sites at positions around Ser 19/Ser 20 and Gly 24. These observations reveal the similar amyloid formation tendency in the C and N terminus segments because of the sequence similarity, while the differences in specific amino acids at each key site manifest the effect of sequence variations. The results could be beneficial for studying structural polymorphism of amyloidal peptides with multiple beta structure motifs.

Original languageEnglish (US)
Pages (from-to)19605-19610
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume108
Issue number49
DOIs
StatePublished - Dec 6 2011
Externally publishedYes

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Islet Amyloid Polypeptide
Scanning Tunnelling Microscopy
Amyloid
Amino Acids
Peptides
islet amyloid polypeptide (8-37)

Keywords

  • Amylin
  • Amyloid
  • Folding sites
  • Self-assembly

ASJC Scopus subject areas

  • General

Cite this

Beta structure motifs of islet amyloid polypeptides identified through surface-mediated assemblies. / Mao, Xiaobo; Wang, Chen Xuan; Wu, Xing Kui; Ma, Xiao Jing; Liu, Lei; Zhang, Lan; Niu, Lin; Guo, Yuan Yuan; Li, Deng Hua; Yang, Yan Lian; Wang, Chen.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, No. 49, 06.12.2011, p. 19605-19610.

Research output: Contribution to journalArticle

Mao, Xiaobo ; Wang, Chen Xuan ; Wu, Xing Kui ; Ma, Xiao Jing ; Liu, Lei ; Zhang, Lan ; Niu, Lin ; Guo, Yuan Yuan ; Li, Deng Hua ; Yang, Yan Lian ; Wang, Chen. / Beta structure motifs of islet amyloid polypeptides identified through surface-mediated assemblies. In: Proceedings of the National Academy of Sciences of the United States of America. 2011 ; Vol. 108, No. 49. pp. 19605-19610.
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AU - Zhang, Lan

AU - Niu, Lin

AU - Guo, Yuan Yuan

AU - Li, Deng Hua

AU - Yang, Yan Lian

AU - Wang, Chen

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