Beta-catenin signaling regulates barrier-specific gene expression in circumventricular organ and ocular vasculatures

Yanshu Wang, Mark F. Sabbagh, Xiaowu Gu, Amir Rattner, John Williams, Jeremy Nathans

Research output: Contribution to journalArticle

Abstract

The brain, spinal cord, and retina are supplied by capillaries that do not permit free diffusion of molecules between serum and parenchyma, a property that defines the blood-brain and blood-retina barriers. Exceptions to this pattern are found in circumventricular organs (CVOs), small midline brain structures that are supplied by high permeability capillaries. In the eye and brain, high permeability capillaries are also present in the choriocapillaris, which supplies the retinal pigment epithelium and photoreceptors, and the ciliary body and choroid plexus, the sources of aqueous humor and cerebrospinal fluid, respectively. We show here that (1) endothelial cells in these high permeability vascular systems have very low beta-catenin signaling compared to barrier-competent endothelial cells, and (2) elevating beta-catenin signaling leads to a partial conversion of permeable endothelial cells to a barrier-type state. In one CVO, the area postrema, high permeability is maintained, in part, by local production of Wnt inhibitory factor-1.

Original languageEnglish (US)
JournaleLife
Volume8
DOIs
StatePublished - Apr 1 2019

Fingerprint

beta Catenin
Capillary Permeability
Gene expression
Brain
Endothelial cells
Endothelial Cells
Gene Expression
Retina
Wnt Proteins
Area Postrema
Ciliary Body
Vertebrate Photoreceptor Cells
Choroid Plexus
Blood
Retinal Pigment Epithelium
Aqueous Humor
Cerebrospinal fluid
Blood-Brain Barrier
Retinal Pigments
Cerebrospinal Fluid

Keywords

  • beta-catenin
  • blood-brain barrier
  • circumventricular organ
  • developmental biology
  • endothelial
  • mouse
  • neuroscience
  • vascular
  • Wnt

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Beta-catenin signaling regulates barrier-specific gene expression in circumventricular organ and ocular vasculatures. / Wang, Yanshu; Sabbagh, Mark F.; Gu, Xiaowu; Rattner, Amir; Williams, John; Nathans, Jeremy.

In: eLife, Vol. 8, 01.04.2019.

Research output: Contribution to journalArticle

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