Abstract
Leptin-deficient Ob/Ob mice are hypometabolic and have reduced fat cell expression of beta-3 adrenoceptors (ARs). To determine whether leptin repletion restores beta-3 AR number, C57BL/6J Ob/Ob mice were given exogenous leptin (5 mg/kg IP daily) for 21 days. Leptin administration reduced body weight from 43.1 ±3.7 to 34.1 ±3.7 g in Ob/Ob animals but had no effect on weight in wildtype animals. Body weight increased by 12% in Ob/Ob mice receiving saline. Beta-3 AR mRNA concentrations were markedly reduced in Ob/Ob animals at baseline. Leptin increased beta-3 AR mRNA to control levels in Ob/Ob mice, but had no effect in wildtype animals. Adipocyte leptin mRNA was increased by 400% in Ob/Ob mice and did not suppress with exogenous leptin administration, suggesting no direct feedback regulation of leptin synthesis. We speculate that restoration of beta-3 AR expression by repleting leptin may be important in correcting hypometabolism in Ob/Ob animals.
Original language | English (US) |
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Pages (from-to) | 59-64 |
Number of pages | 6 |
Journal | Life Sciences |
Volume | 61 |
Issue number | 1 |
DOIs | |
State | Published - May 30 1997 |
Externally published | Yes |
Keywords
- Adrenoceptor
- Beta-3 adrenergic receptor
- Leptin
- Ob/ob mice
- Obesity
- White adipose tissue
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology
- Pharmacology, Toxicology and Pharmaceutics(all)