Benzo(a)pyrene facilitates dermatophagoides group 1 (Der f 1)-induced epithelial cytokine release through aryl hydrocarbon receptor in asthma

Eryi Wang, Xiaoyu Liu, Wei Tu, Danh Do, Haiqiong Yu, Liteng Yang, Yufeng Zhou, Damo Xu, Shau Ku Huang, Pingchang Yang, Pixin Ran, Peisong Gao, Zhigang Liu

Research output: Contribution to journalArticle

Abstract

Background: Environmental pollutants, which coexist with allergens, have been associated with the exacerbation of asthma. However, the underlying molecular mechanisms remain elusive. We sought to determine whether benzo(a)pyrene (BaP) co-exposure with dermatophagoides group 1 allergen (Der f 1) can potentiate Der f 1-induced asthma and its underlying mechanisms. Methods: The effect of BaP was investigated in Der f 1-induced mouse model of asthma, including airway hyper-responsiveness, allergic inflammation, and epithelial-derived cytokines. The impact of BaP on Der f 1-induced airway epithelial cell oxidative stress (ROS) and cytokine release was further analyzed. The role of aryl hydrocarbon receptor (AhR) signaling in BaP-promoted Der f 1-induced ROS, cytokine production, and allergic inflammation was also investigated. Results: Compared with Der f 1, BaP co-exposure with Der f 1 led to airway hyper-responsiveness and increased lung inflammation in mouse model of asthma. Increased expression of TSLP, IL-33, and IL-25 was also found in the airways of these mice. Moreover, BaP co-exposure with Der f 1 activated AhR signaling with increased expression of AhR and CYP1A1 and promoted airway epithelial ROS generation and TSLP and IL-33, but not IL-25, expression. Interestingly, AhR antagonist CH223191 or cells with AhR knockdown abrogated the increased expression of ROS, TSLP, and IL-33. Furthermore, ROS inhibitor N-acetyl-L-cysteine (NAC) also suppressed BaP co-exposure-induced expression of epithelial TSLP, IL-33, and IL-25. Finally, AhR antagonist CH223191 and NAC inhibited BaP co-exposure with Der f 1-induced lung inflammation. Conclusions: Our findings suggest that BaP facilitates Der f 1-induced epithelial cytokine release through the AhR-ROS axis.

Original languageEnglish (US)
Pages (from-to)1675-1690
Number of pages16
JournalAllergy: European Journal of Allergy and Clinical Immunology
Volume74
Issue number9
DOIs
StatePublished - Sep 1 2019

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Aryl Hydrocarbon Receptors
Pyroglyphidae
Benzo(a)pyrene
Asthma
Cytokines
Respiratory Hypersensitivity
Acetylcysteine
Pneumonia
Dermatophagoides farinae antigen f 1
Inflammation
Environmental Pollutants
Cytochrome P-450 CYP1A1
Allergens
Oxidative Stress
Epithelial Cells

Keywords

  • Aryl hydrocarbon receptor
  • benzo(a)pyrene (BaP)
  • dermatophagoides group 1 allergen (Der f 1)
  • IL-25
  • IL-33
  • reactive oxygen species
  • TSLP

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Benzo(a)pyrene facilitates dermatophagoides group 1 (Der f 1)-induced epithelial cytokine release through aryl hydrocarbon receptor in asthma. / Wang, Eryi; Liu, Xiaoyu; Tu, Wei; Do, Danh; Yu, Haiqiong; Yang, Liteng; Zhou, Yufeng; Xu, Damo; Huang, Shau Ku; Yang, Pingchang; Ran, Pixin; Gao, Peisong; Liu, Zhigang.

In: Allergy: European Journal of Allergy and Clinical Immunology, Vol. 74, No. 9, 01.09.2019, p. 1675-1690.

Research output: Contribution to journalArticle

Wang, Eryi ; Liu, Xiaoyu ; Tu, Wei ; Do, Danh ; Yu, Haiqiong ; Yang, Liteng ; Zhou, Yufeng ; Xu, Damo ; Huang, Shau Ku ; Yang, Pingchang ; Ran, Pixin ; Gao, Peisong ; Liu, Zhigang. / Benzo(a)pyrene facilitates dermatophagoides group 1 (Der f 1)-induced epithelial cytokine release through aryl hydrocarbon receptor in asthma. In: Allergy: European Journal of Allergy and Clinical Immunology. 2019 ; Vol. 74, No. 9. pp. 1675-1690.
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abstract = "Background: Environmental pollutants, which coexist with allergens, have been associated with the exacerbation of asthma. However, the underlying molecular mechanisms remain elusive. We sought to determine whether benzo(a)pyrene (BaP) co-exposure with dermatophagoides group 1 allergen (Der f 1) can potentiate Der f 1-induced asthma and its underlying mechanisms. Methods: The effect of BaP was investigated in Der f 1-induced mouse model of asthma, including airway hyper-responsiveness, allergic inflammation, and epithelial-derived cytokines. The impact of BaP on Der f 1-induced airway epithelial cell oxidative stress (ROS) and cytokine release was further analyzed. The role of aryl hydrocarbon receptor (AhR) signaling in BaP-promoted Der f 1-induced ROS, cytokine production, and allergic inflammation was also investigated. Results: Compared with Der f 1, BaP co-exposure with Der f 1 led to airway hyper-responsiveness and increased lung inflammation in mouse model of asthma. Increased expression of TSLP, IL-33, and IL-25 was also found in the airways of these mice. Moreover, BaP co-exposure with Der f 1 activated AhR signaling with increased expression of AhR and CYP1A1 and promoted airway epithelial ROS generation and TSLP and IL-33, but not IL-25, expression. Interestingly, AhR antagonist CH223191 or cells with AhR knockdown abrogated the increased expression of ROS, TSLP, and IL-33. Furthermore, ROS inhibitor N-acetyl-L-cysteine (NAC) also suppressed BaP co-exposure-induced expression of epithelial TSLP, IL-33, and IL-25. Finally, AhR antagonist CH223191 and NAC inhibited BaP co-exposure with Der f 1-induced lung inflammation. Conclusions: Our findings suggest that BaP facilitates Der f 1-induced epithelial cytokine release through the AhR-ROS axis.",
keywords = "Aryl hydrocarbon receptor, benzo(a)pyrene (BaP), dermatophagoides group 1 allergen (Der f 1), IL-25, IL-33, reactive oxygen species, TSLP",
author = "Eryi Wang and Xiaoyu Liu and Wei Tu and Danh Do and Haiqiong Yu and Liteng Yang and Yufeng Zhou and Damo Xu and Huang, {Shau Ku} and Pingchang Yang and Pixin Ran and Peisong Gao and Zhigang Liu",
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T1 - Benzo(a)pyrene facilitates dermatophagoides group 1 (Der f 1)-induced epithelial cytokine release through aryl hydrocarbon receptor in asthma

AU - Wang, Eryi

AU - Liu, Xiaoyu

AU - Tu, Wei

AU - Do, Danh

AU - Yu, Haiqiong

AU - Yang, Liteng

AU - Zhou, Yufeng

AU - Xu, Damo

AU - Huang, Shau Ku

AU - Yang, Pingchang

AU - Ran, Pixin

AU - Gao, Peisong

AU - Liu, Zhigang

PY - 2019/9/1

Y1 - 2019/9/1

N2 - Background: Environmental pollutants, which coexist with allergens, have been associated with the exacerbation of asthma. However, the underlying molecular mechanisms remain elusive. We sought to determine whether benzo(a)pyrene (BaP) co-exposure with dermatophagoides group 1 allergen (Der f 1) can potentiate Der f 1-induced asthma and its underlying mechanisms. Methods: The effect of BaP was investigated in Der f 1-induced mouse model of asthma, including airway hyper-responsiveness, allergic inflammation, and epithelial-derived cytokines. The impact of BaP on Der f 1-induced airway epithelial cell oxidative stress (ROS) and cytokine release was further analyzed. The role of aryl hydrocarbon receptor (AhR) signaling in BaP-promoted Der f 1-induced ROS, cytokine production, and allergic inflammation was also investigated. Results: Compared with Der f 1, BaP co-exposure with Der f 1 led to airway hyper-responsiveness and increased lung inflammation in mouse model of asthma. Increased expression of TSLP, IL-33, and IL-25 was also found in the airways of these mice. Moreover, BaP co-exposure with Der f 1 activated AhR signaling with increased expression of AhR and CYP1A1 and promoted airway epithelial ROS generation and TSLP and IL-33, but not IL-25, expression. Interestingly, AhR antagonist CH223191 or cells with AhR knockdown abrogated the increased expression of ROS, TSLP, and IL-33. Furthermore, ROS inhibitor N-acetyl-L-cysteine (NAC) also suppressed BaP co-exposure-induced expression of epithelial TSLP, IL-33, and IL-25. Finally, AhR antagonist CH223191 and NAC inhibited BaP co-exposure with Der f 1-induced lung inflammation. Conclusions: Our findings suggest that BaP facilitates Der f 1-induced epithelial cytokine release through the AhR-ROS axis.

AB - Background: Environmental pollutants, which coexist with allergens, have been associated with the exacerbation of asthma. However, the underlying molecular mechanisms remain elusive. We sought to determine whether benzo(a)pyrene (BaP) co-exposure with dermatophagoides group 1 allergen (Der f 1) can potentiate Der f 1-induced asthma and its underlying mechanisms. Methods: The effect of BaP was investigated in Der f 1-induced mouse model of asthma, including airway hyper-responsiveness, allergic inflammation, and epithelial-derived cytokines. The impact of BaP on Der f 1-induced airway epithelial cell oxidative stress (ROS) and cytokine release was further analyzed. The role of aryl hydrocarbon receptor (AhR) signaling in BaP-promoted Der f 1-induced ROS, cytokine production, and allergic inflammation was also investigated. Results: Compared with Der f 1, BaP co-exposure with Der f 1 led to airway hyper-responsiveness and increased lung inflammation in mouse model of asthma. Increased expression of TSLP, IL-33, and IL-25 was also found in the airways of these mice. Moreover, BaP co-exposure with Der f 1 activated AhR signaling with increased expression of AhR and CYP1A1 and promoted airway epithelial ROS generation and TSLP and IL-33, but not IL-25, expression. Interestingly, AhR antagonist CH223191 or cells with AhR knockdown abrogated the increased expression of ROS, TSLP, and IL-33. Furthermore, ROS inhibitor N-acetyl-L-cysteine (NAC) also suppressed BaP co-exposure-induced expression of epithelial TSLP, IL-33, and IL-25. Finally, AhR antagonist CH223191 and NAC inhibited BaP co-exposure with Der f 1-induced lung inflammation. Conclusions: Our findings suggest that BaP facilitates Der f 1-induced epithelial cytokine release through the AhR-ROS axis.

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KW - benzo(a)pyrene (BaP)

KW - dermatophagoides group 1 allergen (Der f 1)

KW - IL-25

KW - IL-33

KW - reactive oxygen species

KW - TSLP

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