Behavioral training of marmosets and electrophysiological recording from the cerebellum

Ehsan Sedaghat-Nejad, David J. Herzfeld, Paul Hage, Kaveh Karbasi, Tara Palin, Xiaoqin Wang, Reza Shadmehr

Research output: Contribution to journalArticle

Abstract

The common marmoset (Callithrix jacchus) is a promising new model for study of neurophysiological basis of behavior in primates. Like other primates, it relies on saccadic eye movements to monitor and explore its environment. Previous reports have demonstrated some success in training marmosets to produce goal-directed actions in the laboratory. However, the number of trials per session has been relatively small, thus limiting the utility of marmosets as a model for behavioral and neurophysiological studies. In this article, we report the results of a series of new behavioral training and neurophysiological protocols aimed at increasing the number of trials per session while recording from the cerebellum. To improve the training efficacy, we designed a precisely calibrated food regulation regime that motivates the subjects to perform saccade tasks, resulting in ~1,000 reward-driven trials on a daily basis. We then developed a multichannel recording system that uses imaging to target a desired region of the cerebellum, allowing for simultaneous isolation of multiple Purkinje cells in the vermis. In this report, we describe 1) the design and surgical implantation of a computer tomography (CT)-guided, subject-specific head post, 2) the design of a CT- and MRI-guided alignment tool for trajectory guidance of electrodes mounted on an absolute encoder microdrive, 3) development of a protocol for behavioral training of subjects, and 4) simultaneous recordings from pairs of Purkinje cells during a saccade task.NEW & NOTEWORTHY Marmosets present the opportunity to investigate genetically based neurological disease in primates, in particular, diseases that affect social behaviors, vocal communication, and eye movements. All of these behaviors depend on the integrity of the cerebellum. We present training methods that better motivate the subjects, allowing for improved performance, and we also present electrophysiological techniques that precisely target the subject's cerebellum, allowing for simultaneous isolation of multiple Purkinje cells.

Original languageEnglish (US)
Pages (from-to)1502-1517
Number of pages16
JournalJournal of neurophysiology
Volume122
Issue number4
DOIs
StatePublished - Oct 1 2019

Fingerprint

Callithrix
Cerebellum
Saccades
Purkinje Cells
Primates
Primate Diseases
Tomography
Social Behavior
Eye Movements
Reward
Electrodes
Communication
Head
Food

Keywords

  • cerebellum
  • marmoset
  • saccade
  • surgical methods
  • training methods

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology

Cite this

Behavioral training of marmosets and electrophysiological recording from the cerebellum. / Sedaghat-Nejad, Ehsan; Herzfeld, David J.; Hage, Paul; Karbasi, Kaveh; Palin, Tara; Wang, Xiaoqin; Shadmehr, Reza.

In: Journal of neurophysiology, Vol. 122, No. 4, 01.10.2019, p. 1502-1517.

Research output: Contribution to journalArticle

Sedaghat-Nejad, Ehsan ; Herzfeld, David J. ; Hage, Paul ; Karbasi, Kaveh ; Palin, Tara ; Wang, Xiaoqin ; Shadmehr, Reza. / Behavioral training of marmosets and electrophysiological recording from the cerebellum. In: Journal of neurophysiology. 2019 ; Vol. 122, No. 4. pp. 1502-1517.
@article{702ce66028974777b14ebe767afc10f8,
title = "Behavioral training of marmosets and electrophysiological recording from the cerebellum",
abstract = "The common marmoset (Callithrix jacchus) is a promising new model for study of neurophysiological basis of behavior in primates. Like other primates, it relies on saccadic eye movements to monitor and explore its environment. Previous reports have demonstrated some success in training marmosets to produce goal-directed actions in the laboratory. However, the number of trials per session has been relatively small, thus limiting the utility of marmosets as a model for behavioral and neurophysiological studies. In this article, we report the results of a series of new behavioral training and neurophysiological protocols aimed at increasing the number of trials per session while recording from the cerebellum. To improve the training efficacy, we designed a precisely calibrated food regulation regime that motivates the subjects to perform saccade tasks, resulting in ~1,000 reward-driven trials on a daily basis. We then developed a multichannel recording system that uses imaging to target a desired region of the cerebellum, allowing for simultaneous isolation of multiple Purkinje cells in the vermis. In this report, we describe 1) the design and surgical implantation of a computer tomography (CT)-guided, subject-specific head post, 2) the design of a CT- and MRI-guided alignment tool for trajectory guidance of electrodes mounted on an absolute encoder microdrive, 3) development of a protocol for behavioral training of subjects, and 4) simultaneous recordings from pairs of Purkinje cells during a saccade task.NEW & NOTEWORTHY Marmosets present the opportunity to investigate genetically based neurological disease in primates, in particular, diseases that affect social behaviors, vocal communication, and eye movements. All of these behaviors depend on the integrity of the cerebellum. We present training methods that better motivate the subjects, allowing for improved performance, and we also present electrophysiological techniques that precisely target the subject's cerebellum, allowing for simultaneous isolation of multiple Purkinje cells.",
keywords = "cerebellum, marmoset, saccade, surgical methods, training methods",
author = "Ehsan Sedaghat-Nejad and Herzfeld, {David J.} and Paul Hage and Kaveh Karbasi and Tara Palin and Xiaoqin Wang and Reza Shadmehr",
year = "2019",
month = "10",
day = "1",
doi = "10.1152/jn.00389.2019",
language = "English (US)",
volume = "122",
pages = "1502--1517",
journal = "Journal of Neurophysiology",
issn = "0022-3077",
publisher = "American Physiological Society",
number = "4",

}

TY - JOUR

T1 - Behavioral training of marmosets and electrophysiological recording from the cerebellum

AU - Sedaghat-Nejad, Ehsan

AU - Herzfeld, David J.

AU - Hage, Paul

AU - Karbasi, Kaveh

AU - Palin, Tara

AU - Wang, Xiaoqin

AU - Shadmehr, Reza

PY - 2019/10/1

Y1 - 2019/10/1

N2 - The common marmoset (Callithrix jacchus) is a promising new model for study of neurophysiological basis of behavior in primates. Like other primates, it relies on saccadic eye movements to monitor and explore its environment. Previous reports have demonstrated some success in training marmosets to produce goal-directed actions in the laboratory. However, the number of trials per session has been relatively small, thus limiting the utility of marmosets as a model for behavioral and neurophysiological studies. In this article, we report the results of a series of new behavioral training and neurophysiological protocols aimed at increasing the number of trials per session while recording from the cerebellum. To improve the training efficacy, we designed a precisely calibrated food regulation regime that motivates the subjects to perform saccade tasks, resulting in ~1,000 reward-driven trials on a daily basis. We then developed a multichannel recording system that uses imaging to target a desired region of the cerebellum, allowing for simultaneous isolation of multiple Purkinje cells in the vermis. In this report, we describe 1) the design and surgical implantation of a computer tomography (CT)-guided, subject-specific head post, 2) the design of a CT- and MRI-guided alignment tool for trajectory guidance of electrodes mounted on an absolute encoder microdrive, 3) development of a protocol for behavioral training of subjects, and 4) simultaneous recordings from pairs of Purkinje cells during a saccade task.NEW & NOTEWORTHY Marmosets present the opportunity to investigate genetically based neurological disease in primates, in particular, diseases that affect social behaviors, vocal communication, and eye movements. All of these behaviors depend on the integrity of the cerebellum. We present training methods that better motivate the subjects, allowing for improved performance, and we also present electrophysiological techniques that precisely target the subject's cerebellum, allowing for simultaneous isolation of multiple Purkinje cells.

AB - The common marmoset (Callithrix jacchus) is a promising new model for study of neurophysiological basis of behavior in primates. Like other primates, it relies on saccadic eye movements to monitor and explore its environment. Previous reports have demonstrated some success in training marmosets to produce goal-directed actions in the laboratory. However, the number of trials per session has been relatively small, thus limiting the utility of marmosets as a model for behavioral and neurophysiological studies. In this article, we report the results of a series of new behavioral training and neurophysiological protocols aimed at increasing the number of trials per session while recording from the cerebellum. To improve the training efficacy, we designed a precisely calibrated food regulation regime that motivates the subjects to perform saccade tasks, resulting in ~1,000 reward-driven trials on a daily basis. We then developed a multichannel recording system that uses imaging to target a desired region of the cerebellum, allowing for simultaneous isolation of multiple Purkinje cells in the vermis. In this report, we describe 1) the design and surgical implantation of a computer tomography (CT)-guided, subject-specific head post, 2) the design of a CT- and MRI-guided alignment tool for trajectory guidance of electrodes mounted on an absolute encoder microdrive, 3) development of a protocol for behavioral training of subjects, and 4) simultaneous recordings from pairs of Purkinje cells during a saccade task.NEW & NOTEWORTHY Marmosets present the opportunity to investigate genetically based neurological disease in primates, in particular, diseases that affect social behaviors, vocal communication, and eye movements. All of these behaviors depend on the integrity of the cerebellum. We present training methods that better motivate the subjects, allowing for improved performance, and we also present electrophysiological techniques that precisely target the subject's cerebellum, allowing for simultaneous isolation of multiple Purkinje cells.

KW - cerebellum

KW - marmoset

KW - saccade

KW - surgical methods

KW - training methods

UR - http://www.scopus.com/inward/record.url?scp=85072628516&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85072628516&partnerID=8YFLogxK

U2 - 10.1152/jn.00389.2019

DO - 10.1152/jn.00389.2019

M3 - Article

C2 - 31389752

AN - SCOPUS:85072628516

VL - 122

SP - 1502

EP - 1517

JO - Journal of Neurophysiology

JF - Journal of Neurophysiology

SN - 0022-3077

IS - 4

ER -