BEAMing up for detection and quantification of rare sequence variants

Meng Li, Frank Diehl, Devin Dressman, Bert Vogelstein, Kenneth W. Kinzler

Research output: Contribution to journalArticlepeer-review

200 Scopus citations


BEAMing allows the one-to-one conversion of a population of DNA fragments into a population of beads. We used rolling circle amplification to increase the number of copies bound to such beads by more than 100-fold. This allowed enumeration of mutant and wild-type sequences even when they were present at ratios less than 1:10,000 and was sensitive enough to directly quantify the error rate of DNA polymerases used for PCR.

Original languageEnglish (US)
Pages (from-to)95-97
Number of pages3
JournalNature Methods
Issue number2
StatePublished - Feb 2006

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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