BEAMing up for detection and quantification of rare sequence variants

Meng Li, Frank Diehl, Devin Dressman, Bert Vogelstein, Kenneth W. Kinzler

Research output: Contribution to journalArticle

Abstract

BEAMing allows the one-to-one conversion of a population of DNA fragments into a population of beads. We used rolling circle amplification to increase the number of copies bound to such beads by more than 100-fold. This allowed enumeration of mutant and wild-type sequences even when they were present at ratios less than 1:10,000 and was sensitive enough to directly quantify the error rate of DNA polymerases used for PCR.

Original languageEnglish (US)
Pages (from-to)95-97
Number of pages3
JournalNature Methods
Volume3
Issue number2
DOIs
StatePublished - Feb 1 2006

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'BEAMing up for detection and quantification of rare sequence variants'. Together they form a unique fingerprint.

  • Cite this