BEAMing: Single-molecule PCR on microparticles in water-in-oil emulsions

Frank Diehl, Meng Li, Yiping He, Kenneth W Kinzler, Bert Vogelstein, Devin Dressman

Research output: Contribution to journalArticle

Abstract

The most important biotechnological advances made in the 20th century involved methods that converted single DNA molecules into populations of identical DNA molecules. The first wave of techniques used cells (cloning) and the second used PCR. Cloning was advantageous because the populations arising from individual molecules were inherently separated. In contrast, each template required individual compartments (tubes) for PCR-based methods if separate products were desired. Emulsion PCR overcame this disadvantage by miniaturizing the compartments so that millions of templates could be individually amplified within a single tube3. BEAMing (beads, emulsions, amplification and magnetics) is a process built on emulsion PCR that (i) includes beads within the compartments and (ii) ensures that one strand of the PCR product is bound to the beads. After amplification, each compartment contains a bead that is coated with thousands of copies of the single DNA molecule originally present. These beads can be recovered with a magnet or by centrifugation. Beads obtained via BEAMing accurately reflect the DNA diversity present in template populations and this method can be used to determine what fraction of a DNA population contains a specific mutation. Because each bead contains thousands of molecules of the identical sequence, the signal to noise ratio obtained by hybridization or enzymatic assays is extremely high. Millions of beads can be analyzed within minutes using flow cytometry or optical scanning instruments. The DNA bound to beads also provides excellent templates for high-throughput sequencing. In this protocol we describe detailed methods for BEAMing, including a new technique for simultaneously generating 192 emulsions suitable for BEAMing.

Original languageEnglish (US)
Pages (from-to)551-559
Number of pages9
JournalNature Methods
Volume3
Issue number7
DOIs
StatePublished - Jul 2006

Fingerprint

Magnetics
Emulsions
Oils
Polymerase Chain Reaction
Water
DNA
Population
Organism Cloning
Magnets
Enzyme Assays
Signal-To-Noise Ratio
Centrifugation
Flow Cytometry

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Cell Biology

Cite this

BEAMing : Single-molecule PCR on microparticles in water-in-oil emulsions. / Diehl, Frank; Li, Meng; He, Yiping; Kinzler, Kenneth W; Vogelstein, Bert; Dressman, Devin.

In: Nature Methods, Vol. 3, No. 7, 07.2006, p. 551-559.

Research output: Contribution to journalArticle

Diehl, Frank ; Li, Meng ; He, Yiping ; Kinzler, Kenneth W ; Vogelstein, Bert ; Dressman, Devin. / BEAMing : Single-molecule PCR on microparticles in water-in-oil emulsions. In: Nature Methods. 2006 ; Vol. 3, No. 7. pp. 551-559.
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