BDNF-TrkB signaling in oxytocin neurons contributes to maternal behavior

Kristen R. Maynard, John W. Hobbs, Ba Doi N. Phan, Amolika Gupta, Sumita Rajpurohit, Courtney Williams, Anandita Rajpurohit, Joo Heon Shin, Andrew Jaffe, Keri Martinowich

Research output: Contribution to journalArticle

Abstract

Brain-derived neurotrophic factor (Bdnf) transcription is controlled by several promoters, which drive expression of multiple transcripts encoding an identical protein. We previously reported that BDNF derived from promoters I and II is highly expressed in hypothalamus and is critical for regulating aggression in male mice. Here we report that BDNF loss from these promoters causes reduced sexual receptivity and impaired maternal care in female mice, which is concomitant with decreased oxytocin (Oxt) expression during development. We identify a novel link between BDNF signaling, oxytocin, and maternal behavior by demonstrating that ablation of TrkB selectively in OXT neurons partially recapitulates maternal care impairments observed in BDNF-deficient females. Using translating ribosome affinity purification and RNA-sequencing we define a molecular profile for OXT neurons and delineate how BDNF signaling impacts gene pathways critical for structural and functional plasticity. Our findings highlight BDNF as a modulator of sexually-dimorphic hypothalamic circuits that govern female-typical behaviors.

Original languageEnglish (US)
JournaleLife
Volume7
DOIs
StatePublished - Sep 7 2018

Fingerprint

Maternal Behavior
Brain-Derived Neurotrophic Factor
Oxytocin
Neurons
Mothers
RNA Sequence Analysis
Critical Pathways
Transcription
Ablation
Aggression
Ribosomes
Modulators
Hypothalamus
Purification
Plasticity
Genes
RNA
Networks (circuits)

Keywords

  • brain-derived neurotrophic factor
  • hypothalamus
  • maternal care
  • mouse
  • neuroscience
  • neurotrophins
  • oxytocin
  • translating ribosome affinity purification

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Maynard, K. R., Hobbs, J. W., Phan, B. D. N., Gupta, A., Rajpurohit, S., Williams, C., ... Martinowich, K. (2018). BDNF-TrkB signaling in oxytocin neurons contributes to maternal behavior. eLife, 7. https://doi.org/10.7554/eLife.33676

BDNF-TrkB signaling in oxytocin neurons contributes to maternal behavior. / Maynard, Kristen R.; Hobbs, John W.; Phan, Ba Doi N.; Gupta, Amolika; Rajpurohit, Sumita; Williams, Courtney; Rajpurohit, Anandita; Shin, Joo Heon; Jaffe, Andrew; Martinowich, Keri.

In: eLife, Vol. 7, 07.09.2018.

Research output: Contribution to journalArticle

Maynard, KR, Hobbs, JW, Phan, BDN, Gupta, A, Rajpurohit, S, Williams, C, Rajpurohit, A, Shin, JH, Jaffe, A & Martinowich, K 2018, 'BDNF-TrkB signaling in oxytocin neurons contributes to maternal behavior', eLife, vol. 7. https://doi.org/10.7554/eLife.33676
Maynard KR, Hobbs JW, Phan BDN, Gupta A, Rajpurohit S, Williams C et al. BDNF-TrkB signaling in oxytocin neurons contributes to maternal behavior. eLife. 2018 Sep 7;7. https://doi.org/10.7554/eLife.33676
Maynard, Kristen R. ; Hobbs, John W. ; Phan, Ba Doi N. ; Gupta, Amolika ; Rajpurohit, Sumita ; Williams, Courtney ; Rajpurohit, Anandita ; Shin, Joo Heon ; Jaffe, Andrew ; Martinowich, Keri. / BDNF-TrkB signaling in oxytocin neurons contributes to maternal behavior. In: eLife. 2018 ; Vol. 7.
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