BCL11B expression in intramembranous osteogenesis during murine craniofacial suture development

Greg Holmes, Harm Van Bakel, Xueyan Zhou, Bojan Losic, Ethylin Wang Jabs

Research output: Contribution to journalArticle

Abstract

Sutures, where neighboring craniofacial bones are separated by undifferentiated mesenchyme, are major growth sites during craniofacial development. Pathologic fusion of bones within sutures occurs in a wide variety of craniosynostosis conditions and can result in dysmorphic craniofacial growth and secondary neurologic deficits. Our knowledge of the genes involved in suture formation is poor. Here we describe the novel expression pattern of the BCL11B transcription factor protein during murine embryonic craniofacial bone formation. We examined BCL11B protein expression at E14.5, E16.5, and E18.5 in 14 major craniofacial sutures of C57BL/6J mice. We found BCL11B expression to be associated with all intramembranous craniofacial bones examined. The most striking aspects of BCL11B expression were its high levels in suture mesenchyme and increasingly complementary expression with RUNX2 in differentiating osteoblasts during development. BCL11B was also expressed in mesenchyme at the non-sutural edges of intramembranous bones. No expression was seen in osteoblasts involved in endochondral ossification of the cartilaginous cranial base. BCL11B is expressed to potentially regulate the transition of mesenchymal differentiation and suture formation within craniofacial intramembranous bone.

Original languageEnglish (US)
Pages (from-to)16-25
Number of pages10
JournalGene Expression Patterns
Volume17
Issue number1
DOIs
StatePublished - 2015
Externally publishedYes

Fingerprint

Osteogenesis
Sutures
Bone and Bones
Mesoderm
Osteoblasts
Craniosynostoses
Skull Base
Growth
Neurologic Manifestations
Inbred C57BL Mouse
Proteins
Transcription Factors
Genes

Keywords

  • Bone
  • Calvaria
  • Ctip2
  • Differentiation
  • Osteoblasts
  • Skull

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Developmental Biology

Cite this

BCL11B expression in intramembranous osteogenesis during murine craniofacial suture development. / Holmes, Greg; Van Bakel, Harm; Zhou, Xueyan; Losic, Bojan; Jabs, Ethylin Wang.

In: Gene Expression Patterns, Vol. 17, No. 1, 2015, p. 16-25.

Research output: Contribution to journalArticle

Holmes, Greg ; Van Bakel, Harm ; Zhou, Xueyan ; Losic, Bojan ; Jabs, Ethylin Wang. / BCL11B expression in intramembranous osteogenesis during murine craniofacial suture development. In: Gene Expression Patterns. 2015 ; Vol. 17, No. 1. pp. 16-25.
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