TY - JOUR
T1 - bcl-2 Alters the Antigen-Driven Selection of B Cells in μκ but Not in μ-Only Xid Transgenic Mice
AU - Kenny, James J.
AU - Fischer, Randy T.
AU - Lustig, Ana
AU - Dintzis, Howard
AU - Katsumata, Makoto
AU - Reed, John C.
AU - Longo, Dan L.
PY - 1996/8/1
Y1 - 1996/8/1
N2 - A point mutation in the pleckstrin homology domain of the mouse Bruton's tyrosine kinase (btk) gene results in an X-linked immune defect, Xid, characterized by immunologic unresponsiveness to polymeric carbohydrate Ags. In Xid mice, B cells specific for phosphocholine (PC) do not develop in peripheral lymphoid tissues because they either fail to be positively selected from the marrow or they are clonally deleted via an Ag-driven, receptor-mediated process. Overexpression of the bcl-2 gene allows PC-specific B cells to survive and mature in Xid μκ anti-PC transgenic mice, but PC-specific B cells are not rescued by bcl-2 in Xid μ-only transgenic mice. The failure of bcl-2 to rescue PC-specific B cells in μ-only transgenic mice suggests that either it does not correct the btk defect in the Ag-driven selection process that occurs in pre-B cells and/or in very immature B cells or that a btk-dependent proliferative phase is required for the selection and amplification of the PC-specific B cells in μ-only transgenic mice. The rescue of PC-specific B cells in μκ transgenic mice indicates that bcl-2 can alter receptor-mediated B cell selection at late stages in B cell development. The rescued PC-specific B cells in Xid male mice do not exhibit an altered proliferation profile in response to B cell-stimulating agents compared with B cells from unmanipulated Xid mice; thus, they fail to respond to soluble anti-μ or PC-dextran, but they proliferate in response to PC, anti-μ, or anti-Id conjugated to Sepharose.
AB - A point mutation in the pleckstrin homology domain of the mouse Bruton's tyrosine kinase (btk) gene results in an X-linked immune defect, Xid, characterized by immunologic unresponsiveness to polymeric carbohydrate Ags. In Xid mice, B cells specific for phosphocholine (PC) do not develop in peripheral lymphoid tissues because they either fail to be positively selected from the marrow or they are clonally deleted via an Ag-driven, receptor-mediated process. Overexpression of the bcl-2 gene allows PC-specific B cells to survive and mature in Xid μκ anti-PC transgenic mice, but PC-specific B cells are not rescued by bcl-2 in Xid μ-only transgenic mice. The failure of bcl-2 to rescue PC-specific B cells in μ-only transgenic mice suggests that either it does not correct the btk defect in the Ag-driven selection process that occurs in pre-B cells and/or in very immature B cells or that a btk-dependent proliferative phase is required for the selection and amplification of the PC-specific B cells in μ-only transgenic mice. The rescue of PC-specific B cells in μκ transgenic mice indicates that bcl-2 can alter receptor-mediated B cell selection at late stages in B cell development. The rescued PC-specific B cells in Xid male mice do not exhibit an altered proliferation profile in response to B cell-stimulating agents compared with B cells from unmanipulated Xid mice; thus, they fail to respond to soluble anti-μ or PC-dextran, but they proliferate in response to PC, anti-μ, or anti-Id conjugated to Sepharose.
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M3 - Article
C2 - 8757609
AN - SCOPUS:0030209818
SN - 0022-1767
VL - 157
SP - 1054
EP - 1061
JO - Journal of Immunology
JF - Journal of Immunology
IS - 3
ER -