Basophil activation testing

Research output: Contribution to journalArticle

Abstract

Both the treatment of patients with allergic diseases and the study of allergic disease mechanisms depend on a wide variety of assays that in various ways assess the presence and function of IgE antibody. The study of allergic diseases could benefit from the study of its 2 principle cellular participants, mast cells and basophils, but the basophil is more accessible than mast cells for ex vivo studies. Its functionality is tested by using 2 predominant methodologies: the secretion of mediators of allergic inflammation and the expression of proteins on the plasma membrane after stimulation. Each approach has benefits. There are also many operational details to consider regardless of which general approach is taken, and proper interpretation of the methods requires a good understanding of the reagents used and the receptors expressed on basophils and a detailed understanding of the factors regulating aggregation of cell-surface IgE.

Original languageEnglish (US)
Pages (from-to)777-787
Number of pages11
JournalThe Journal of Allergy and Clinical Immunology
Volume132
Issue number4
DOIs
StatePublished - Oct 2013

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Basophils
Mast Cells
Immunoglobulin E
Inflammation Mediators
Cell Membrane
Antibodies
Proteins
Therapeutics

Keywords

  • basophil
  • IgE antibody

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Basophil activation testing. / Macglashan, Donald.

In: The Journal of Allergy and Clinical Immunology, Vol. 132, No. 4, 10.2013, p. 777-787.

Research output: Contribution to journalArticle

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