Basic characteristics of human lung mast cell desensitization

Donald Macglashan, L. M. Lichtenstein

Research output: Contribution to journalArticle

Abstract

Human lung parenchymal mast cells displayed both specific and nonspecific desensitization. The kinetics of both release and desensitization were ~3 times faster than human basophils, but a similar relationship between release and desensitization suggests similar biochemistries in basophils and mast cells. Arachidonic acid metabolite (PGD2 and LTC4) release was slower to desensitize (t( 1/2 ) of 8 min) than histamine release (t( 1/2 ) of 3 min), the ratio of which is similar to the ratio observed in basophils. Ionophore A23187-induced release was unaffected by desensitization to anti-IgE antibody, and calcium-45 uptake was inhibited by desensitization, suggesting that desensitization inhibits the early post-cross-linking 'influx' of calcium that is necessary for mediator release in mast cells. In contrast to the above similarities in basophil and mast cell desensitization, mast cell desensitization, unlike that of basophils, was not inhibited by diisopropylfluorophosphate.

Original languageEnglish (US)
Pages (from-to)501-505
Number of pages5
JournalJournal of Immunology
Volume139
Issue number2
StatePublished - 1987

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Basophils
Mast Cells
Lung
Calcium
Isoflurophate
Prostaglandin D2
Leukotriene C4
Histamine Release
Ionophores
Calcimycin
Arachidonic Acid
Biochemistry

ASJC Scopus subject areas

  • Immunology

Cite this

Basic characteristics of human lung mast cell desensitization. / Macglashan, Donald; Lichtenstein, L. M.

In: Journal of Immunology, Vol. 139, No. 2, 1987, p. 501-505.

Research output: Contribution to journalArticle

Macglashan, Donald ; Lichtenstein, L. M. / Basic characteristics of human lung mast cell desensitization. In: Journal of Immunology. 1987 ; Vol. 139, No. 2. pp. 501-505.
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