Basal-like breast cancer displays distinct patterns of promoter methylation

Ji Shin Lee, Mary Jo Fackler, Jae Hyuk Lee, Chan Choi, Min Ho Park, Jung Han Yoon, Zhe Zhang, Saraswati Sukumar

Research output: Contribution to journalArticlepeer-review

Abstract

Recent microarray profiling studies on breast cancer have identified distinct subtypes that are associated with different clinical outcomes. promoter hypermethylation of several known or putative tumor suppressor genes occurs frequently during the pathogenesis of breast cancer. We proposed that immunohistopathologic subtypes of breast cancer are likely to contain distinct promoter methylation patterns. a panel of 10 gene promoters was assessed by quantitative multiplex methylation-specific PCR in 114 invasive ductal carcinomas from Korea representing the three major subtypes [57 luminal, 24 human epidermal growth factor 2 (HER2), and 33 basal-like] based on immunohistochemical findings of estrogen receptor, progesterone receptor, HER2, cytokeratin 5/6, and epidermal growth factor receptor. The median methylation levels of HIN1, RASSF1A and TWIST, and the average methylation ratio were significantly lower in basal-like subtype compared to luminal or HER2 subtypes. In contrast, BRCA1 methylation level was significantly higher in basal-like subtype than in luminal subtype. The methylation status of a panel of four genes (APC1, CDH, BRCA1 and RAR-β) in luminal and HER2 subtypes were dissimilar, where HER2 tumors showed a significantly higher level of methylation compared to luminal tumors. These results suggest that gene methylation in breast cancer can potentially serve as epigenetic biomarkers and may contribute further to current breast cancer classification.

Original languageEnglish (US)
Pages (from-to)1017-1024
Number of pages8
JournalCancer Biology and Therapy
Volume9
Issue number12
DOIs
StatePublished - Jun 15 2010

Keywords

  • Basal-like
  • Breast cancer
  • HER2
  • Luminal
  • Methylation
  • Quantitation

ASJC Scopus subject areas

  • Molecular Medicine
  • Oncology
  • Pharmacology
  • Cancer Research

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