Basal DNA damage in individual human lymphocytes with age

Narendra P. Singh; David B. Danner; Raymond R. Tice; Jay D. Pearson; Larry J. Brant; Chris H. Morrell; Edward L. Schneider

doi:10.1016/0921-8734(91)90026-8

Basal DNA damage in individual human lymphocytes with age

Narendra P. Singh, David B. Danner, Raymond R. Tice, Jay D. Pearson, Larry J. Brant, Chris H. Morrell, Edward L. Schneider

Research output: Contribution to journal › Article › peer-review

107 Scopus citations

Abstract

A role for DNA damage is central to many theories of aging, but attempts to show an increase in DNA damage with age have yielded contradictory results. However, previous experiments have been of limited sensitivity, only able to examine induced (not basal) damage or pooled (not individual) cells. In this report, we apply a novel technique (Singh et al., 1988) to directly measure basal levels of DNA single-strand breaks and alkali-labile sites in individual human peripheral blood lymphocytes (PBL) obtained from young (less than 60 years) and old (more than 60 years) male donors. This approach shows that while average changes with age are small, changes in certain individuals and in certain cells may be large: the mean increase in damage was only 12%, but the increase in a subpopulation of highly damaged lymphocytes was 5-fold. However, most of this increase was contributed by just 3 of 17 older subjects. Further characterization of these individuals may shed light on the relationship between DNA damage and aging.

Original language	English (US)
Pages (from-to)	1-6
Number of pages	6
Journal	Mutation Research DNAging
Volume	256
Issue number	1
DOIs	https://doi.org/10.1016/0921-8734(91)90026-8
State	Published - Jan 1991
Externally published	Yes

Keywords

Basal DNA damage
Human lymphocytes
Single-cell gel electrophoresis

ASJC Scopus subject areas

Aging
Molecular Biology
Genetics

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10.1016/0921-8734(91)90026-8

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title = "Basal DNA damage in individual human lymphocytes with age",

abstract = "A role for DNA damage is central to many theories of aging, but attempts to show an increase in DNA damage with age have yielded contradictory results. However, previous experiments have been of limited sensitivity, only able to examine induced (not basal) damage or pooled (not individual) cells. In this report, we apply a novel technique (Singh et al., 1988) to directly measure basal levels of DNA single-strand breaks and alkali-labile sites in individual human peripheral blood lymphocytes (PBL) obtained from young (less than 60 years) and old (more than 60 years) male donors. This approach shows that while average changes with age are small, changes in certain individuals and in certain cells may be large: the mean increase in damage was only 12%, but the increase in a subpopulation of highly damaged lymphocytes was 5-fold. However, most of this increase was contributed by just 3 of 17 older subjects. Further characterization of these individuals may shed light on the relationship between DNA damage and aging.",

keywords = "Basal DNA damage, Human lymphocytes, Single-cell gel electrophoresis",

author = "Singh, {Narendra P.} and Danner, {David B.} and Tice, {Raymond R.} and Pearson, {Jay D.} and Brant, {Larry J.} and Morrell, {Chris H.} and Schneider, {Edward L.}",

note = "Funding Information: We are grateful to Howard Baldwin, Mary E. Bannon, and Faye Barrack for collecting blood samples and to Megan E. Greene for help with statistical analysis. We appreciate the use of equipment belonging to Drs. Raymond Saltero and Donald Lightfoot, and the photographic assistance of Derrick Hanson. We thank Dr. Peter Cerutti for extensive discussions and expert advice. N.P.S. was supported by the Glen Foundation for Medical Research and by the Donald H. Yarborough Charity Fund. R.R.T. is supported at Brookhaven National Laboratory by the Department of Energy under prime contract DE-AC02-76CH00016.",

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language = "English (US)",

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AU - Morrell, Chris H.

AU - Schneider, Edward L.

N1 - Funding Information: We are grateful to Howard Baldwin, Mary E. Bannon, and Faye Barrack for collecting blood samples and to Megan E. Greene for help with statistical analysis. We appreciate the use of equipment belonging to Drs. Raymond Saltero and Donald Lightfoot, and the photographic assistance of Derrick Hanson. We thank Dr. Peter Cerutti for extensive discussions and expert advice. N.P.S. was supported by the Glen Foundation for Medical Research and by the Donald H. Yarborough Charity Fund. R.R.T. is supported at Brookhaven National Laboratory by the Department of Energy under prime contract DE-AC02-76CH00016.

PY - 1991/1

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N2 - A role for DNA damage is central to many theories of aging, but attempts to show an increase in DNA damage with age have yielded contradictory results. However, previous experiments have been of limited sensitivity, only able to examine induced (not basal) damage or pooled (not individual) cells. In this report, we apply a novel technique (Singh et al., 1988) to directly measure basal levels of DNA single-strand breaks and alkali-labile sites in individual human peripheral blood lymphocytes (PBL) obtained from young (less than 60 years) and old (more than 60 years) male donors. This approach shows that while average changes with age are small, changes in certain individuals and in certain cells may be large: the mean increase in damage was only 12%, but the increase in a subpopulation of highly damaged lymphocytes was 5-fold. However, most of this increase was contributed by just 3 of 17 older subjects. Further characterization of these individuals may shed light on the relationship between DNA damage and aging.

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Basal DNA damage in individual human lymphocytes with age

Abstract

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