BARX2 induces cadherin 6 expression and is a functional suppressor of ovarian cancer progression

Grant C. Sellar, Li Li, Karen P. Watt, Barry D. Nelkin, Genevieve J. Rabiasz, Euan A. Stronach, Eric P. Miller, David J. Porteous, John F. Smyth, Hani Gabra

Research output: Contribution to journalArticlepeer-review

Abstract

The human homeobox gene BARX2 is located at 11q24-q25, within a minimal region associated with frequent loss of heterozygosity and adverse survival in epithelial ovarian cancer. BARX2 is a transcription factor that regulates transcription of specific cell adhesion molecules in the mouse. We show that BARX2 and cadherin 6 are expressed in normal human ovarian surface epithelium. BARX2 and cadherin 6 both have significantly lower expression in a clinical sample of endometrioid and clear cell ovarian cancers, as compared with serous or mixed mesodermal tumors. In a series of ovarian cancer cell lines, BARX2 expression showed a significant direct correlation with cadherin 6 expression. In OAW42, an ovarian cancer cell line that does not endogenously express BARX2, in vitro transfection of human BARX2 cDNA induced cadherin 6 expression. Transfection of BARX2 into OAW42 inhibited Matrigel invasion, haptotactic cellular migration to a collagen IV signal, and adhesion to collagen IV-coated plates. Our data demonstrate that BARX2 is expressed in the ovarian surface epithelium and has functional suppressor properties in ovarian cancer cells.

Original languageEnglish (US)
Pages (from-to)6977-6981
Number of pages5
JournalCancer Research
Volume61
Issue number19
StatePublished - Oct 1 2001

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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