Barrier-to-autointegration factor-like (BAF-L): A proposed regulator of BAF

Kathryn E. Tifft, Miriam Segura-Totten, Kenneth K. Lee, Katherine L. Wilson

Research output: Contribution to journalArticle

Abstract

Barrier-to-autointegration factor (BAF) is an essential chromatin protein conserved in metazoans. BAF has roles in nuclear assembly, chromatin organization, gene expression, and gonad development and is exploited by retroviruses. BAF forms stable dimers that bind nonspecifically to dsDNA and specifically to LEM-domain proteins (e.g., LAP2β, emerin, MAN1), homeodomain transcription factors, histones, and lamin A. We characterized a protein named BAF-Like (BAF-L) that in humans is 40% identical to BAF. Overexpression studies in HeLa cells show that BAF-L, like BAF, is a predominantly nuclear protein. Recombinant BAF-L forms stable homodimers and heterodimerizes with BAF in vitro and also interacts with BAF in vivo. BAF-L does not bind significantly to DNA, LAP2β, or emerin but can form ternary complexes in vitro with BAF plus DNA, or BAF plus LAP2β. Levels of BAF-L mRNA were high in pancreas and testis, suggesting functions in the germline. BAF-L mRNA was detectable at low levels in eleven other tissues and undetectable in heart and skeletal muscle which are specifically affected by Emery-Dreifuss muscular dystrophy, a disease caused by mutations in either emerin or lamin A. We propose that BAF-L regulates BAF function via heterodimerization and might thereby influence tissue-specific roles of BAF.

Original languageEnglish (US)
Pages (from-to)478-487
Number of pages10
JournalExperimental cell research
Volume312
Issue number4
DOIs
StatePublished - Feb 15 2006

Keywords

  • Barrier-to-autointegration factor
  • Emerin
  • Emery-Dreifuss muscular dystrophy
  • Germline
  • LEM-domain
  • Lamina associated polypeptide 2β
  • Nuclear envelope
  • Testis

ASJC Scopus subject areas

  • Cell Biology

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