Barrel pattern formation requires serotonin uptake by thalamocortical afferents, and not vesicular monoamine release

Antonio M. Persico, Elisa Mengual, Rainald Moessner, Scott F. Hall, Randal S. Revay, Ichiro Sora, Jon Arellano, Javier DeFelipe, José Manuel Giménez-Amaya, Monica Conciatori, Ramona Marino, Alfonso Baldi, Simona Cabib, Tiziana Pascucci, George R. Uhl, Dennis L. Murphy, K. Peter Lesch, Flavio Keller

Research output: Contribution to journalArticle

Abstract

Thalamocortical neurons innervating the barrel cortex in neonatal rodents transiently store serotonin (5-HT) in synaptic vesicles by expressing the plasma membrane serotonin transporter (5-HTT) and the vesicular monoamine transporter (VMAT2). 5-HTT knock-out (ko) mice reveal a nearly complete absence of 5-HT in the cerebral cortex by immunohistochemistry, and of barrels, both at P7 and adulthood. Quantitative electron microscopy reveals that 5-HTT ko affects neither the density of synapses nor the length of synaptic contacts in layer IV. VMAT2 ko mice, completely lacking activity-dependent vesicular release of monoamines including 5-HT, also show a complete lack of 5-HT in the cortex but display largely normal barrel fields, despite sometimes markedly reduced postnatal growth. Transient 5-HTT expression is thus required for barrel pattern formation, whereas activity-dependent vesicular 5-HT release is not.

Original languageEnglish (US)
Pages (from-to)6862-6873
Number of pages12
JournalJournal of Neuroscience
Volume21
Issue number17
StatePublished - Sep 1 2001
Externally publishedYes

Fingerprint

Serotonin
Knockout Mice
Vesicular Monoamine Transport Proteins
Serotonin Plasma Membrane Transport Proteins
Membrane Transport Proteins
Synaptic Vesicles
Cerebral Cortex
Synapses
Rodentia
Electron Microscopy
Immunohistochemistry
Cell Membrane
Neurons
Growth

Keywords

  • Barrel
  • GABA transporter
  • Homologous recombination
  • Knock-out
  • Monoamine
  • p-chlorophenylalanine
  • Serotonin
  • Serotonin transporter
  • Vesicular monoamine transporter
  • Whisker

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Persico, A. M., Mengual, E., Moessner, R., Hall, S. F., Revay, R. S., Sora, I., ... Keller, F. (2001). Barrel pattern formation requires serotonin uptake by thalamocortical afferents, and not vesicular monoamine release. Journal of Neuroscience, 21(17), 6862-6873.

Barrel pattern formation requires serotonin uptake by thalamocortical afferents, and not vesicular monoamine release. / Persico, Antonio M.; Mengual, Elisa; Moessner, Rainald; Hall, Scott F.; Revay, Randal S.; Sora, Ichiro; Arellano, Jon; DeFelipe, Javier; Giménez-Amaya, José Manuel; Conciatori, Monica; Marino, Ramona; Baldi, Alfonso; Cabib, Simona; Pascucci, Tiziana; Uhl, George R.; Murphy, Dennis L.; Peter Lesch, K.; Keller, Flavio.

In: Journal of Neuroscience, Vol. 21, No. 17, 01.09.2001, p. 6862-6873.

Research output: Contribution to journalArticle

Persico, AM, Mengual, E, Moessner, R, Hall, SF, Revay, RS, Sora, I, Arellano, J, DeFelipe, J, Giménez-Amaya, JM, Conciatori, M, Marino, R, Baldi, A, Cabib, S, Pascucci, T, Uhl, GR, Murphy, DL, Peter Lesch, K & Keller, F 2001, 'Barrel pattern formation requires serotonin uptake by thalamocortical afferents, and not vesicular monoamine release', Journal of Neuroscience, vol. 21, no. 17, pp. 6862-6873.
Persico AM, Mengual E, Moessner R, Hall SF, Revay RS, Sora I et al. Barrel pattern formation requires serotonin uptake by thalamocortical afferents, and not vesicular monoamine release. Journal of Neuroscience. 2001 Sep 1;21(17):6862-6873.
Persico, Antonio M. ; Mengual, Elisa ; Moessner, Rainald ; Hall, Scott F. ; Revay, Randal S. ; Sora, Ichiro ; Arellano, Jon ; DeFelipe, Javier ; Giménez-Amaya, José Manuel ; Conciatori, Monica ; Marino, Ramona ; Baldi, Alfonso ; Cabib, Simona ; Pascucci, Tiziana ; Uhl, George R. ; Murphy, Dennis L. ; Peter Lesch, K. ; Keller, Flavio. / Barrel pattern formation requires serotonin uptake by thalamocortical afferents, and not vesicular monoamine release. In: Journal of Neuroscience. 2001 ; Vol. 21, No. 17. pp. 6862-6873.
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