Banded structures in collagen vitrigels for corneal injury repair

Zhiyong Xia, Xiomara Calderón-Colón, Russell McCally, Jeffrey Maranchi, Lixia Rong, Benjamin Hsiao, Jennifer Hartt Elisseeff, Morgana Trexler

Research output: Contribution to journalArticle

Abstract

There is a growing interest in using collagen vitrigels for corneal injury repair. We recently reported the synthesis and thermal denaturation behavior of these gels. In this paper, the banded structure in these vitrified gels is studied by small-angle X-ray scattering (SAXS) one-dimensional (1-D) correlation function analysis and transmission electron microscopy (TEM). Results demonstrate that the collagen vitrigel possess banded structures similar to those of the starting type I collagen, with an average D-spacing of 64 nm (by SAXS) or 57 nm (by TEM). A combination of SAXS 1-D correlation function analyses and TEM show that overlap and gap distances ranged from 30 to 33 nm and from 23 to 25 nm, respectively. Changing the vitrification condition does not impact on the banded structure significantly.

Original languageEnglish (US)
Pages (from-to)3615-3619
Number of pages5
JournalActa Biomaterialia
Volume10
Issue number8
DOIs
StatePublished - 2014

Fingerprint

X ray scattering
Transmission Electron Microscopy
Collagen
Repair
X-Rays
Transmission electron microscopy
Gels
Vitrification
Denaturation
Collagen Type I
Hot Temperature
Corneal Injuries

Keywords

  • Banded structure
  • collagen vitrigel
  • Corneal injury repair
  • D-Spacing
  • Small-angle X-ray scattering

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Medicine(all)

Cite this

Xia, Z., Calderón-Colón, X., McCally, R., Maranchi, J., Rong, L., Hsiao, B., ... Trexler, M. (2014). Banded structures in collagen vitrigels for corneal injury repair. Acta Biomaterialia, 10(8), 3615-3619. https://doi.org/10.1016/j.actbio.2014.05.010

Banded structures in collagen vitrigels for corneal injury repair. / Xia, Zhiyong; Calderón-Colón, Xiomara; McCally, Russell; Maranchi, Jeffrey; Rong, Lixia; Hsiao, Benjamin; Elisseeff, Jennifer Hartt; Trexler, Morgana.

In: Acta Biomaterialia, Vol. 10, No. 8, 2014, p. 3615-3619.

Research output: Contribution to journalArticle

Xia, Z, Calderón-Colón, X, McCally, R, Maranchi, J, Rong, L, Hsiao, B, Elisseeff, JH & Trexler, M 2014, 'Banded structures in collagen vitrigels for corneal injury repair', Acta Biomaterialia, vol. 10, no. 8, pp. 3615-3619. https://doi.org/10.1016/j.actbio.2014.05.010
Xia Z, Calderón-Colón X, McCally R, Maranchi J, Rong L, Hsiao B et al. Banded structures in collagen vitrigels for corneal injury repair. Acta Biomaterialia. 2014;10(8):3615-3619. https://doi.org/10.1016/j.actbio.2014.05.010
Xia, Zhiyong ; Calderón-Colón, Xiomara ; McCally, Russell ; Maranchi, Jeffrey ; Rong, Lixia ; Hsiao, Benjamin ; Elisseeff, Jennifer Hartt ; Trexler, Morgana. / Banded structures in collagen vitrigels for corneal injury repair. In: Acta Biomaterialia. 2014 ; Vol. 10, No. 8. pp. 3615-3619.
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