Abstract
Cytokinesis, the fission of a mother cell into two daughter cells, is a simple and dramatic cell shape change. Here, we examine the dynamics of cytokinesis by using a combination of microscopy, dynamic measurements, and genetic analysis. We find that cytokinesis proceeds through a single sequence of shape changes, but the kinetics of the transformation from one shape to another differs dramatically between strains. We interpret the measurements in a simple and quantitative manner by using a previously uncharacterized analytic model. From the analysis, wild-type cytokinesis appears to proceed through an active, extremely regulated process in which globally distributed proteins generate resistive forces that slow the rate of furrow ingression. Finally, we propose that, in addition to myosin II, a Laplace pressure, resulting from material properties and the geometry of the dividing cell, generates force to help drive furrow ingression late in cytokinesis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 7186-7191 |
| Number of pages | 6 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 102 |
| Issue number | 20 |
| DOIs | |
| State | Published - May 17 2005 |
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Keywords
- Cell dynamics
- Cell mechanics
- Dynacortin
- Myosin II
- RacE
ASJC Scopus subject areas
- Genetics
- General
Cite this
Balance of actively generated contractile and resistive forces controls cytokinesis dynamics. / Zhang, Wendy; Robinson, Douglas.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, No. 20, 17.05.2005, p. 7186-7191.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Balance of actively generated contractile and resistive forces controls cytokinesis dynamics
AU - Zhang, Wendy
AU - Robinson, Douglas
PY - 2005/5/17
Y1 - 2005/5/17
N2 - Cytokinesis, the fission of a mother cell into two daughter cells, is a simple and dramatic cell shape change. Here, we examine the dynamics of cytokinesis by using a combination of microscopy, dynamic measurements, and genetic analysis. We find that cytokinesis proceeds through a single sequence of shape changes, but the kinetics of the transformation from one shape to another differs dramatically between strains. We interpret the measurements in a simple and quantitative manner by using a previously uncharacterized analytic model. From the analysis, wild-type cytokinesis appears to proceed through an active, extremely regulated process in which globally distributed proteins generate resistive forces that slow the rate of furrow ingression. Finally, we propose that, in addition to myosin II, a Laplace pressure, resulting from material properties and the geometry of the dividing cell, generates force to help drive furrow ingression late in cytokinesis.
AB - Cytokinesis, the fission of a mother cell into two daughter cells, is a simple and dramatic cell shape change. Here, we examine the dynamics of cytokinesis by using a combination of microscopy, dynamic measurements, and genetic analysis. We find that cytokinesis proceeds through a single sequence of shape changes, but the kinetics of the transformation from one shape to another differs dramatically between strains. We interpret the measurements in a simple and quantitative manner by using a previously uncharacterized analytic model. From the analysis, wild-type cytokinesis appears to proceed through an active, extremely regulated process in which globally distributed proteins generate resistive forces that slow the rate of furrow ingression. Finally, we propose that, in addition to myosin II, a Laplace pressure, resulting from material properties and the geometry of the dividing cell, generates force to help drive furrow ingression late in cytokinesis.
KW - Cell dynamics
KW - Cell mechanics
KW - Dynacortin
KW - Myosin II
KW - RacE
UR - http://www.scopus.com/inward/record.url?scp=18844414788&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=18844414788&partnerID=8YFLogxK
U2 - 10.1073/pnas.0502545102
DO - 10.1073/pnas.0502545102
M3 - Article
C2 - 15870188
AN - SCOPUS:18844414788
VL - 102
SP - 7186
EP - 7191
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 20
ER -