BAF is required for emerin assembly into the reforming nuclear envelope

Tokuko Haraguchi, Takako Koujin, Miriam Segura-Totten, Kenneth K. Lee, Yosuke Matsuoka, Yoshihiro Yoneda, Katherine L. Wilson, Yasushi Hiraoka

Research output: Contribution to journalArticlepeer-review

Abstract

Mutations in emerin cause the X-linked recessive form of Emery-Dreifuss muscular dystrophy (EDMD). Emerin localizes at the inner membrane of the nuclear envelope (NE) during interphase, and diffuses into the ER when the NE disassembles during mitosis. We analyzed the recruitment of wildtype and mutant GFP-tagged emerin proteins during nuclear envelope assembly in living HeLa cells. During telophase, emerin accumulates briefly at the 'core' region of telophase chromosomes, and later distributes over the entire nuclear rim. Barrier-to-autointegration factor (BAF), a protein that binds nonspecifically to double-stranded DNA in vitro, colocalized with emerin at the 'core' region of chromosomes during telophase. An emerin mutant defective for binding to BAF in vitro failed to localize at the 'core' in vivo, and subsequently failed to localize at the reformed NE. In HeLa cells that expressed BAF mutant G25E, which did not show 'core' localization, the endogenous emerin proteins failed to localize at the 'core' region during telophase, and did not assemble into the NE during the subsequent interphase. BAF mutant G25E also dominantly dislocalized LAP2β and lamin A from the NE, but had no effect on the localization of lamin B. We conclude that BAF is required for the assembly of emerin and A-type lamins at the reforming NE during telophase, and may mediate their stability in the subsequent interphase.

Original languageEnglish (US)
Pages (from-to)4575-4585
Number of pages11
JournalJournal of cell science
Volume114
Issue number24
StatePublished - 2001
Externally publishedYes

Keywords

  • Barrier-to-autointegration factor
  • Chromosome
  • Emery-Dreifuss muscular dystrophy
  • Lamin A
  • Lamin-associated polypeptide 2
  • MAN1
  • Nuclear envelope

ASJC Scopus subject areas

  • Cell Biology

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