Bacteroides fragilis toxin 2 damages human colonic mucosa in vitro

M. Riegler, M. Lotz, Cynthia Louise Sears, C. Pothoulakis, I. Castagliuolo, C. C. Wang, R. Sedivy, T. Sogukoglu, E. Cosentini, G. Bischof, W. Feil, B. Teleky, G. Hamilton, J. T. LaMont, E. Wenzl

Research output: Contribution to journalArticle

Abstract

Background - Strains of Bacteroides fragilis producing a 20 kDa protein toxin (B fragilis toxin (BFT) or fragilysin) are associated with diarrhoea in animals and humans. Although in vitro results indicate that BFT damages intestinal epithelial cells in culture, the effects of BFT on native human colon are not known. Aims - To examine the electrophysiological and morphological effects of purified BFT-2 on human colonic mucosa in vitro. Methods - For resistance (R) measurements, colonic mucosa mounted in Ussing chambers was exposed to luminal or serosal BFT-2 (1.25-10 nM) and after four hours morphological damage was measured on haematoxylin and eosin stained sections using morphometry. F actin distribution was assessed using confocal microscopy. Results - Serosal BFT-2 for four hours was four-, two-, seven-, and threefold more potent than luminal BFT-2 in decreasing resistance, increasing epithelial 3H-mannitol permeability, and damaging crypt and surface colonocytes, respectively (p

Original languageEnglish (US)
Pages (from-to)504-510
Number of pages7
JournalGut
Volume44
Issue number4
StatePublished - 1999

Fingerprint

Mucous Membrane
Bacteroides fragilis
Mannitol
Hematoxylin
Eosine Yellowish-(YS)
Population Groups
Confocal Microscopy
Actins
Diarrhea
Permeability
Colon
Cell Culture Techniques
Epithelial Cells
Bacteroides fragilis toxin
In Vitro Techniques
fragilysin
IgA receptor

Keywords

  • Actin filaments
  • B fragilis toxin
  • Morphometry
  • Toxin mediated colonocyte damage
  • Transepithelial resistance

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Riegler, M., Lotz, M., Sears, C. L., Pothoulakis, C., Castagliuolo, I., Wang, C. C., ... Wenzl, E. (1999). Bacteroides fragilis toxin 2 damages human colonic mucosa in vitro. Gut, 44(4), 504-510.

Bacteroides fragilis toxin 2 damages human colonic mucosa in vitro. / Riegler, M.; Lotz, M.; Sears, Cynthia Louise; Pothoulakis, C.; Castagliuolo, I.; Wang, C. C.; Sedivy, R.; Sogukoglu, T.; Cosentini, E.; Bischof, G.; Feil, W.; Teleky, B.; Hamilton, G.; LaMont, J. T.; Wenzl, E.

In: Gut, Vol. 44, No. 4, 1999, p. 504-510.

Research output: Contribution to journalArticle

Riegler, M, Lotz, M, Sears, CL, Pothoulakis, C, Castagliuolo, I, Wang, CC, Sedivy, R, Sogukoglu, T, Cosentini, E, Bischof, G, Feil, W, Teleky, B, Hamilton, G, LaMont, JT & Wenzl, E 1999, 'Bacteroides fragilis toxin 2 damages human colonic mucosa in vitro', Gut, vol. 44, no. 4, pp. 504-510.
Riegler M, Lotz M, Sears CL, Pothoulakis C, Castagliuolo I, Wang CC et al. Bacteroides fragilis toxin 2 damages human colonic mucosa in vitro. Gut. 1999;44(4):504-510.
Riegler, M. ; Lotz, M. ; Sears, Cynthia Louise ; Pothoulakis, C. ; Castagliuolo, I. ; Wang, C. C. ; Sedivy, R. ; Sogukoglu, T. ; Cosentini, E. ; Bischof, G. ; Feil, W. ; Teleky, B. ; Hamilton, G. ; LaMont, J. T. ; Wenzl, E. / Bacteroides fragilis toxin 2 damages human colonic mucosa in vitro. In: Gut. 1999 ; Vol. 44, No. 4. pp. 504-510.
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AU - Wang, C. C.

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AU - Cosentini, E.

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AU - Feil, W.

AU - Teleky, B.

AU - Hamilton, G.

AU - LaMont, J. T.

AU - Wenzl, E.

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