TY - JOUR
T1 - Bacterial-sized particle inflow through sutured clear corneal incisions in a laboratory human model
AU - May, William N.
AU - Castro-Combs, Juan
AU - Kashiwabuchi, Renata T.
AU - Hertzog, Hans
AU - Tattiyakul, Woranart
AU - Kahn, Yasin A.
AU - Hirai, Flavio
AU - Gower, Emily W.
AU - Behrens, Ashley
N1 - Funding Information:
Supported by the May Vision Foundation , Los Angeles, California, an unrestricted grant from Research to Prevent Blindness , New York, New York, to Wilmer Eye Institute of the Johns Hopkins University School of Medicine, the Research to Prevent Blindness Althouse Special Scholars Award and Fight for Sight, New York, New York, USA.
Copyright:
Copyright 2011 Elsevier B.V., All rights reserved.
PY - 2011/6
Y1 - 2011/6
N2 - Purpose: To determine the effectiveness of a single radial suture placement in 2 clear corneal incision (CCI) configurations in preventing inflow of bacterial-sized particles. Setting: Wilmer Eye Institute, Baltimore, Maryland, USA. Design: Experimental study. Methods: Ten human globes were used. Two 25-gauge needles connected to a saline solution bag and to a digital manometer were inserted through the limbus 120 degrees apart. Four incision-suture combinations were evaluated sequentially in each cornea. All incisions had the same dimensions (2.75 mm width, 3.00 mm length). Two incisions were single plane, and 2 were biplane. One incision from each configuration was left unsutured, and the other was sutured (10-0 nylon). With a preset 10 mm Hg intraocular pressure (IOP), India ink was applied to the incision site and a sudden IOP fluctuation was induced. Inflow was outlined and measured by planimetry. Results: There was a significant increase in area and linear distance of India-ink inflow after pressure challenge in all groups (P<.05), but with important differences among them. When the sutured and unsutured groups from each CCI were compared, there was a significantly smaller area of inflow in the 2-step unsutured group (P<.05). The linear inflow was higher in both sutured groups; however, the difference was significant for the 2-step incision configuration only (P<.05). Conclusions: A single radial suture reduced the area of inflow of bacterial-sized particles but increased the linear distance of inflow in single-plane 3.0 mm incisions. Suture placement in a stepped incision increased inflow of bacterial-sized particles. Financial Disclosure: No author has a financial or proprietary interest in any material or method mentioned.
AB - Purpose: To determine the effectiveness of a single radial suture placement in 2 clear corneal incision (CCI) configurations in preventing inflow of bacterial-sized particles. Setting: Wilmer Eye Institute, Baltimore, Maryland, USA. Design: Experimental study. Methods: Ten human globes were used. Two 25-gauge needles connected to a saline solution bag and to a digital manometer were inserted through the limbus 120 degrees apart. Four incision-suture combinations were evaluated sequentially in each cornea. All incisions had the same dimensions (2.75 mm width, 3.00 mm length). Two incisions were single plane, and 2 were biplane. One incision from each configuration was left unsutured, and the other was sutured (10-0 nylon). With a preset 10 mm Hg intraocular pressure (IOP), India ink was applied to the incision site and a sudden IOP fluctuation was induced. Inflow was outlined and measured by planimetry. Results: There was a significant increase in area and linear distance of India-ink inflow after pressure challenge in all groups (P<.05), but with important differences among them. When the sutured and unsutured groups from each CCI were compared, there was a significantly smaller area of inflow in the 2-step unsutured group (P<.05). The linear inflow was higher in both sutured groups; however, the difference was significant for the 2-step incision configuration only (P<.05). Conclusions: A single radial suture reduced the area of inflow of bacterial-sized particles but increased the linear distance of inflow in single-plane 3.0 mm incisions. Suture placement in a stepped incision increased inflow of bacterial-sized particles. Financial Disclosure: No author has a financial or proprietary interest in any material or method mentioned.
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U2 - 10.1016/j.jcrs.2010.11.042
DO - 10.1016/j.jcrs.2010.11.042
M3 - Article
C2 - 21497050
AN - SCOPUS:79956216195
SN - 0886-3350
VL - 37
SP - 1140
EP - 1146
JO - Journal of cataract and refractive surgery
JF - Journal of cataract and refractive surgery
IS - 6
ER -