Bacterial cell wall composition, lysozyme resistance, and the induction of chronic arthritis in rats

T. J A Lehman, J. B. Allen, P. H. Plotz, R. L. Wilder

Research output: Contribution to journalArticle

Abstract

Bacterial cell wall induced arthritis is an experimental model of chronic erosive synovitis in which arthritis is induced in rats by a single injection of an aqueous suspension of cell wall fragments from selected Grampositive bacteria. To understand better the Gram-positive bacterial cell wall characteristics necessary for the induction of chronic arthritis we tested the arthritogenicity of five Gram-positive bacteria which were (1) lysozyme resistant and contained a polyrhamnose peptidoglycan side chain moiety, (2) lysozyme resistant, but had little or no rhamnose in the peptidoglycan, polysaccharide, or (3) neither lysozyme resistant, nor contained rhamnose in their peptidoglycan, polysaccharide. All of the lysozyme resistant cell walls tested induced acute arthritis, but only those cell walls which were both lysozyme resistant and contained rhamnose in their polysaccharide side chain were able to induce chronic arthritis. Cell walls which were neither lysozyme resistant nor contained rhamnose were not arthritogenic. These data suggest that both lysozyme resistance and the rhamnose moiety in the peptidoglycan, polysaccharide side chain play an important role in the induction of chronic arthritis by Gram-positive bacterial cell walls in aqueous suspension.

Original languageEnglish (US)
Pages (from-to)163-167
Number of pages5
JournalRheumatology International
Volume5
Issue number4
DOIs
StatePublished - Jul 1985
Externally publishedYes

Fingerprint

Muramidase
Cell Wall
Rhamnose
Arthritis
Peptidoglycan
Polysaccharides
Suspensions
Synovitis
Gram-Positive Bacteria
Theoretical Models
Bacteria
Injections

Keywords

  • Bacterial cell wall-induced arthritis
  • Lactobacillus casei
  • Lysozyme
  • Rats
  • Rhamnose
  • Streptococcus pyogenes

ASJC Scopus subject areas

  • Rheumatology

Cite this

Bacterial cell wall composition, lysozyme resistance, and the induction of chronic arthritis in rats. / Lehman, T. J A; Allen, J. B.; Plotz, P. H.; Wilder, R. L.

In: Rheumatology International, Vol. 5, No. 4, 07.1985, p. 163-167.

Research output: Contribution to journalArticle

Lehman, T. J A ; Allen, J. B. ; Plotz, P. H. ; Wilder, R. L. / Bacterial cell wall composition, lysozyme resistance, and the induction of chronic arthritis in rats. In: Rheumatology International. 1985 ; Vol. 5, No. 4. pp. 163-167.
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