Bacillus Spore Identification via Proteolytic Peptide Mapping with a Miniaturized MALDI TOF Mass Spectrometer

Robert D. English, Bettina Warscheid, Catherine Fenselau, Robert J. Cotter

Research output: Contribution to journalArticlepeer-review

Abstract

An approach is tested here as a rapid screening method for Bacillus spore species employing bacterial peptide analysis with a miniaturized MALDI TOF mass spectrometer. A limited set of tryptic peptides was generated in situ following selective solubilization of the small, acid-soluble protein family (SASP) from spore samples on the MALDI sample holder. To facilitate species identification, a compact database was created comprising masses of the tryptic cleavage products generated in silico from all Bacillus and Clostridium SASPs whose sequences are available in public databases. Experimental measurements were matched against the custom-made database, and a published statistical model was then used to evaluate the probability of false identifications.

Original languageEnglish (US)
Pages (from-to)6886-6893
Number of pages8
JournalAnalytical chemistry
Volume75
Issue number24
DOIs
StatePublished - Dec 15 2003

ASJC Scopus subject areas

  • Analytical Chemistry

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