B7-H1 costimulation preferentially enhances CD28-independent T-helper cell function

Hideto Tamura, Haidong Dong, Gefeng Zhu, Gabriel L. Sica, Dallas B. Flies, Koji Tamada, Lieping Chen

Research output: Contribution to journalArticle

Abstract

B7-H1 is a recently described B7-like molecule that costimulates T-cell growth and cytokine secretion without binding to CD28, cytotoxic T-lymphocyte antigen-4 (CTLA-4), and inducible costimulator (ICOS). In this report, a mouse homologue of human B7-H1 is identified, and its immunologic functions are studied in vitro and in vivo. Mouse B7-H1 shares 69% amino acid homology to the human counterpart. Similar to human B7-H1, mouse B7-H1 can be induced to express on macrophages, T cells, and B cells and to enhance T-cell proliferation and secretion of interleukin-10 (IL-10), interferon-γ, and granulocyte-macrophage colony-stimulating factor but not IL-2 and IL-4. Furthermore, B7-H1 preferentially costimulates CD4+ T cells independently of CD28 and enhances mixed lymphocyte responses to allogeneic antigens. In contrast to B7-1, expression of B7-H1 on murine P815 tumor cells by transfection fails to increase allogeneic and syngeneic cytolytic T-cell responses in vitro and in vivo. Administration of B7-H1lg fusion protein, however, enhances keyhole limpet hemocyanin-specific T-cell proliferation and 2,4,6-trinitrophenyl-specific immunoglobulin G2a antibody production. The study thus identifies a unique costimulatory pathway that preferentially affects T-helper cell functions.

Original languageEnglish (US)
Pages (from-to)1809-1816
Number of pages8
JournalBlood
Volume97
Issue number6
DOIs
StatePublished - Mar 15 2001
Externally publishedYes

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T-cells
Helper-Inducer T-Lymphocytes
T-Lymphocytes
Cell proliferation
Inducible T-Cell Co-Stimulator Protein
Cells
Cell Proliferation
B7 Antigens
CTLA-4 Antigen
Lymphocytes
Macrophages
Cell growth
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-4
Interleukin-10
Interferons
Antibody Formation
Interleukin-2
Transfection
Immunoglobulins

ASJC Scopus subject areas

  • Hematology

Cite this

Tamura, H., Dong, H., Zhu, G., Sica, G. L., Flies, D. B., Tamada, K., & Chen, L. (2001). B7-H1 costimulation preferentially enhances CD28-independent T-helper cell function. Blood, 97(6), 1809-1816. https://doi.org/10.1182/blood.V97.6.1809

B7-H1 costimulation preferentially enhances CD28-independent T-helper cell function. / Tamura, Hideto; Dong, Haidong; Zhu, Gefeng; Sica, Gabriel L.; Flies, Dallas B.; Tamada, Koji; Chen, Lieping.

In: Blood, Vol. 97, No. 6, 15.03.2001, p. 1809-1816.

Research output: Contribution to journalArticle

Tamura, H, Dong, H, Zhu, G, Sica, GL, Flies, DB, Tamada, K & Chen, L 2001, 'B7-H1 costimulation preferentially enhances CD28-independent T-helper cell function', Blood, vol. 97, no. 6, pp. 1809-1816. https://doi.org/10.1182/blood.V97.6.1809
Tamura H, Dong H, Zhu G, Sica GL, Flies DB, Tamada K et al. B7-H1 costimulation preferentially enhances CD28-independent T-helper cell function. Blood. 2001 Mar 15;97(6):1809-1816. https://doi.org/10.1182/blood.V97.6.1809
Tamura, Hideto ; Dong, Haidong ; Zhu, Gefeng ; Sica, Gabriel L. ; Flies, Dallas B. ; Tamada, Koji ; Chen, Lieping. / B7-H1 costimulation preferentially enhances CD28-independent T-helper cell function. In: Blood. 2001 ; Vol. 97, No. 6. pp. 1809-1816.
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