B-lymphocytes and co-stimulatory molecules in Mycobacterium tuberculosis infection

Mercè Corominas, V. Cardona, L. Gonzalez, J. A. Caylà, G. Rufi, M. Mestre, E. Buendia

Research output: Contribution to journalArticle

Abstract

SETTING: The immunological mechanisms that lead to the control of Mycobacterium tuberculosis infection are not well known. OBJECTIVE: To study the role of lymphocyte subsets and co-stimulatory molecules in M. tuberculosis infection. DESIGN: In 35 patients with pulmonary tuberculosis (PTB) and their contacts, 29 persons with tuberculin skin test conversion (TSTC) and 20 healthy individuals with negative tuberculin skin test (NTST), we studied T-lymphocyte subsets (CD3, CD4, CD8, αβTCR and γδTCR), B-cells, monocytes and co-stimulatory molecules CD28 and CD86 in peripheral blood. The results were analysed at univariate and multivariate level through discriminant analysis. RESULTS: At univariate level, compared with TSTC and NTST, PTB patients presented a decrease in CD4+ T-cells (P = 0.002), and B-cells (P = 0. 02 and 0.001, respectively). With regard to NTST subjects, PTB patients also showed a decrease in the percentage of CD86+ monocytes (P = 0.02) and an increase in the percentage of CD86+ B-lymphocytes (P = 0.02). At multivariate level, CD4+ T-lymphocytes showed statistical differences between PTB and TSTC subjects (P = 0.001). B-lymphocytes were discriminant between PTB and NTST (P <0.001) and between TSTC and NTST individuals (P = 0.01). CONCLUSION: The number of total CD4+ T-cells is the best discriminant parameter for distinguishing between disease and infection, whereas the B-cell count is the best between healthy and infected individuals.

Original languageEnglish (US)
Pages (from-to)98-105
Number of pages8
JournalInternational Journal of Tuberculosis and Lung Disease
Volume8
Issue number1
StatePublished - Jan 2004
Externally publishedYes

Fingerprint

Tuberculin Test
Mycobacterium Infections
Skin Tests
Mycobacterium tuberculosis
B-Lymphocytes
Pulmonary Tuberculosis
T-Lymphocytes
Monocytes
Lymphocyte Subsets
T-Lymphocyte Subsets
Discriminant Analysis
Cell Count

Keywords

  • B-cells
  • Cellular immunity
  • Co-stimulatory molecules
  • Lymphocyte subsets
  • Tuberculosis

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Corominas, M., Cardona, V., Gonzalez, L., Caylà, J. A., Rufi, G., Mestre, M., & Buendia, E. (2004). B-lymphocytes and co-stimulatory molecules in Mycobacterium tuberculosis infection. International Journal of Tuberculosis and Lung Disease, 8(1), 98-105.

B-lymphocytes and co-stimulatory molecules in Mycobacterium tuberculosis infection. / Corominas, Mercè; Cardona, V.; Gonzalez, L.; Caylà, J. A.; Rufi, G.; Mestre, M.; Buendia, E.

In: International Journal of Tuberculosis and Lung Disease, Vol. 8, No. 1, 01.2004, p. 98-105.

Research output: Contribution to journalArticle

Corominas, M, Cardona, V, Gonzalez, L, Caylà, JA, Rufi, G, Mestre, M & Buendia, E 2004, 'B-lymphocytes and co-stimulatory molecules in Mycobacterium tuberculosis infection', International Journal of Tuberculosis and Lung Disease, vol. 8, no. 1, pp. 98-105.
Corominas M, Cardona V, Gonzalez L, Caylà JA, Rufi G, Mestre M et al. B-lymphocytes and co-stimulatory molecules in Mycobacterium tuberculosis infection. International Journal of Tuberculosis and Lung Disease. 2004 Jan;8(1):98-105.
Corominas, Mercè ; Cardona, V. ; Gonzalez, L. ; Caylà, J. A. ; Rufi, G. ; Mestre, M. ; Buendia, E. / B-lymphocytes and co-stimulatory molecules in Mycobacterium tuberculosis infection. In: International Journal of Tuberculosis and Lung Disease. 2004 ; Vol. 8, No. 1. pp. 98-105.
@article{6a1a6bfcd51b4aa58529183e55c37197,
title = "B-lymphocytes and co-stimulatory molecules in Mycobacterium tuberculosis infection",
abstract = "SETTING: The immunological mechanisms that lead to the control of Mycobacterium tuberculosis infection are not well known. OBJECTIVE: To study the role of lymphocyte subsets and co-stimulatory molecules in M. tuberculosis infection. DESIGN: In 35 patients with pulmonary tuberculosis (PTB) and their contacts, 29 persons with tuberculin skin test conversion (TSTC) and 20 healthy individuals with negative tuberculin skin test (NTST), we studied T-lymphocyte subsets (CD3, CD4, CD8, αβTCR and γδTCR), B-cells, monocytes and co-stimulatory molecules CD28 and CD86 in peripheral blood. The results were analysed at univariate and multivariate level through discriminant analysis. RESULTS: At univariate level, compared with TSTC and NTST, PTB patients presented a decrease in CD4+ T-cells (P = 0.002), and B-cells (P = 0. 02 and 0.001, respectively). With regard to NTST subjects, PTB patients also showed a decrease in the percentage of CD86+ monocytes (P = 0.02) and an increase in the percentage of CD86+ B-lymphocytes (P = 0.02). At multivariate level, CD4+ T-lymphocytes showed statistical differences between PTB and TSTC subjects (P = 0.001). B-lymphocytes were discriminant between PTB and NTST (P <0.001) and between TSTC and NTST individuals (P = 0.01). CONCLUSION: The number of total CD4+ T-cells is the best discriminant parameter for distinguishing between disease and infection, whereas the B-cell count is the best between healthy and infected individuals.",
keywords = "B-cells, Cellular immunity, Co-stimulatory molecules, Lymphocyte subsets, Tuberculosis",
author = "Merc{\`e} Corominas and V. Cardona and L. Gonzalez and Cayl{\`a}, {J. A.} and G. Rufi and M. Mestre and E. Buendia",
year = "2004",
month = "1",
language = "English (US)",
volume = "8",
pages = "98--105",
journal = "International Journal of Tuberculosis and Lung Disease",
issn = "1027-3719",
publisher = "International Union against Tubercul. and Lung Dis.",
number = "1",

}

TY - JOUR

T1 - B-lymphocytes and co-stimulatory molecules in Mycobacterium tuberculosis infection

AU - Corominas, Mercè

AU - Cardona, V.

AU - Gonzalez, L.

AU - Caylà, J. A.

AU - Rufi, G.

AU - Mestre, M.

AU - Buendia, E.

PY - 2004/1

Y1 - 2004/1

N2 - SETTING: The immunological mechanisms that lead to the control of Mycobacterium tuberculosis infection are not well known. OBJECTIVE: To study the role of lymphocyte subsets and co-stimulatory molecules in M. tuberculosis infection. DESIGN: In 35 patients with pulmonary tuberculosis (PTB) and their contacts, 29 persons with tuberculin skin test conversion (TSTC) and 20 healthy individuals with negative tuberculin skin test (NTST), we studied T-lymphocyte subsets (CD3, CD4, CD8, αβTCR and γδTCR), B-cells, monocytes and co-stimulatory molecules CD28 and CD86 in peripheral blood. The results were analysed at univariate and multivariate level through discriminant analysis. RESULTS: At univariate level, compared with TSTC and NTST, PTB patients presented a decrease in CD4+ T-cells (P = 0.002), and B-cells (P = 0. 02 and 0.001, respectively). With regard to NTST subjects, PTB patients also showed a decrease in the percentage of CD86+ monocytes (P = 0.02) and an increase in the percentage of CD86+ B-lymphocytes (P = 0.02). At multivariate level, CD4+ T-lymphocytes showed statistical differences between PTB and TSTC subjects (P = 0.001). B-lymphocytes were discriminant between PTB and NTST (P <0.001) and between TSTC and NTST individuals (P = 0.01). CONCLUSION: The number of total CD4+ T-cells is the best discriminant parameter for distinguishing between disease and infection, whereas the B-cell count is the best between healthy and infected individuals.

AB - SETTING: The immunological mechanisms that lead to the control of Mycobacterium tuberculosis infection are not well known. OBJECTIVE: To study the role of lymphocyte subsets and co-stimulatory molecules in M. tuberculosis infection. DESIGN: In 35 patients with pulmonary tuberculosis (PTB) and their contacts, 29 persons with tuberculin skin test conversion (TSTC) and 20 healthy individuals with negative tuberculin skin test (NTST), we studied T-lymphocyte subsets (CD3, CD4, CD8, αβTCR and γδTCR), B-cells, monocytes and co-stimulatory molecules CD28 and CD86 in peripheral blood. The results were analysed at univariate and multivariate level through discriminant analysis. RESULTS: At univariate level, compared with TSTC and NTST, PTB patients presented a decrease in CD4+ T-cells (P = 0.002), and B-cells (P = 0. 02 and 0.001, respectively). With regard to NTST subjects, PTB patients also showed a decrease in the percentage of CD86+ monocytes (P = 0.02) and an increase in the percentage of CD86+ B-lymphocytes (P = 0.02). At multivariate level, CD4+ T-lymphocytes showed statistical differences between PTB and TSTC subjects (P = 0.001). B-lymphocytes were discriminant between PTB and NTST (P <0.001) and between TSTC and NTST individuals (P = 0.01). CONCLUSION: The number of total CD4+ T-cells is the best discriminant parameter for distinguishing between disease and infection, whereas the B-cell count is the best between healthy and infected individuals.

KW - B-cells

KW - Cellular immunity

KW - Co-stimulatory molecules

KW - Lymphocyte subsets

KW - Tuberculosis

UR - http://www.scopus.com/inward/record.url?scp=1642516163&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1642516163&partnerID=8YFLogxK

M3 - Article

C2 - 14974752

AN - SCOPUS:1642516163

VL - 8

SP - 98

EP - 105

JO - International Journal of Tuberculosis and Lung Disease

JF - International Journal of Tuberculosis and Lung Disease

SN - 1027-3719

IS - 1

ER -