B cells from young and old mice switch isotypes with equal frequencies after ex vivo stimulation

Lisa M. Russell Knode, Han Sol Park, Robert W. Maul, Patricia J. Gearhart

Research output: Contribution to journalArticlepeer-review

Abstract

To determine whether old B cells have the same capacity to switch isotypes as young cells, we purified splenic follicular, marginal zone, and age-associated B cell subsets from C57BL/6 mice. Cells were stimulated in culture with interleukin 4 and either lipopolysaccharide or anti-CD40, and switching to IgG1 was measured by flow cytometry of surface immunoglobulin. The results show that switching was robust in follicular and marginal zone B cells from old mice and was comparable to their young counterparts. However, age-associated B cells from old mice switched poorly relative to the other subsets. Expression of activation-induced deaminase, which initiates switching, was quantified by qPCR of mRNA, and it was equal between young and old follicular B cells. Thus, in this ex vivo system, the follicular and marginal zone cells from young and old mice behaved similarly, showing that the molecular machinery to perform switching is intact in old B cells.

Original languageEnglish (US)
Article number103966
JournalCellular Immunology
Volume345
DOIs
StatePublished - Nov 2019
Externally publishedYes

Keywords

  • Activation-induced deaminase
  • Age
  • B cell subsets
  • Class switch recombination
  • Ex vivo stimulation
  • Mice

ASJC Scopus subject areas

  • Immunology

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