TY - JOUR
T1 - B cells from young and old mice switch isotypes with equal frequencies after ex vivo stimulation
AU - Russell Knode, Lisa M.
AU - Park, Han Sol
AU - Maul, Robert W.
AU - Gearhart, Patricia J.
N1 - Funding Information:
All animal protocols were reviewed and approved by the Animal Care and Use Committee of the National Institute on Aging. This work was supported entirely through the Intramural Research Program at the National Institutes of Health , National Institute on Aging ( AG000777 ).
Publisher Copyright:
© 2019
PY - 2019/11
Y1 - 2019/11
N2 - To determine whether old B cells have the same capacity to switch isotypes as young cells, we purified splenic follicular, marginal zone, and age-associated B cell subsets from C57BL/6 mice. Cells were stimulated in culture with interleukin 4 and either lipopolysaccharide or anti-CD40, and switching to IgG1 was measured by flow cytometry of surface immunoglobulin. The results show that switching was robust in follicular and marginal zone B cells from old mice and was comparable to their young counterparts. However, age-associated B cells from old mice switched poorly relative to the other subsets. Expression of activation-induced deaminase, which initiates switching, was quantified by qPCR of mRNA, and it was equal between young and old follicular B cells. Thus, in this ex vivo system, the follicular and marginal zone cells from young and old mice behaved similarly, showing that the molecular machinery to perform switching is intact in old B cells.
AB - To determine whether old B cells have the same capacity to switch isotypes as young cells, we purified splenic follicular, marginal zone, and age-associated B cell subsets from C57BL/6 mice. Cells were stimulated in culture with interleukin 4 and either lipopolysaccharide or anti-CD40, and switching to IgG1 was measured by flow cytometry of surface immunoglobulin. The results show that switching was robust in follicular and marginal zone B cells from old mice and was comparable to their young counterparts. However, age-associated B cells from old mice switched poorly relative to the other subsets. Expression of activation-induced deaminase, which initiates switching, was quantified by qPCR of mRNA, and it was equal between young and old follicular B cells. Thus, in this ex vivo system, the follicular and marginal zone cells from young and old mice behaved similarly, showing that the molecular machinery to perform switching is intact in old B cells.
KW - Activation-induced deaminase
KW - Age
KW - B cell subsets
KW - Class switch recombination
KW - Ex vivo stimulation
KW - Mice
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U2 - 10.1016/j.cellimm.2019.103966
DO - 10.1016/j.cellimm.2019.103966
M3 - Article
C2 - 31447053
AN - SCOPUS:85071080575
SN - 0008-8749
VL - 345
JO - Cellular Immunology
JF - Cellular Immunology
M1 - 103966
ER -