B cells and plasma cells in coronaries of chronically rejected cardiac transplants

Jennifer R. Wehner, Karen Fox-Talbot, Marc K Halushka, Carla Ellis, Andrea A. Zachary, William M. Baldwin

Research output: Contribution to journalArticle

Abstract

BACKGROUND: Previously, we reported that transcripts of immunoglobulins were increased in coronary arteries dissected from cardiac transplants with arteriopathy, but the prevelance and patterns of B cell and plasma cell infiltration in cardiac allografts has not been documented. METHODS: In this study, we documented the frequency and distribution of B cells and plasma cells in 16 cardiac transplants with advanced chronic rejection that were explanted during a second transplant procedure. Coronary arteries with pathologically confirmed allograft vasculopathy and controls with native atherosclerosis were immunohistologically stained for markers of T cells, B cells, plasma cells, IgG subclasses, C4d, CD21, and CXCL13. RESULTS: We found that B cells and plasma cells were prevalent in most of the samples analyzed (14 of 16) and were distributed in three patterns: adventitial nodules, diffuse adventitial infiltrates, and neointimal infiltrates. These cells were found most frequently in nodules, some of which had distinct compartmentalization and granular C4d deposits on follicular dendritic cells (FDCs) that typify tertiary lymphoid nodules. FDCs also stained for CD21 and CXCL13. Diffuse infiltrates of B cells and plasma cells were found in fibrotic areas of the neointima and adventitia. Only a minority of control coronaries with atherosclerosis contained B cells. CONCLUSIONS: B cells and plasma cell infiltrates are consistent findings in and around coronary arteries with allograft vasculopathy and are significantly more frequent than in coronaries with native atherosclerosis. The presence of C4d on FDCs in tertiary lymphoid nodules suggests active antigen presentation.

Original languageEnglish (US)
Pages (from-to)1141-1148
Number of pages8
JournalTransplantation
Volume89
Issue number9
DOIs
StatePublished - May 2010

Fingerprint

Plasma Cells
B-Lymphocytes
Transplants
Follicular Dendritic Cells
Adventitia
Allografts
Coronary Vessels
Atherosclerosis
Neointima
Antigen Presentation
Immunoglobulins
Coronary Artery Disease
Immunoglobulin G
T-Lymphocytes

Keywords

  • B cells
  • Chronic allograft vasculopathy
  • Plasma cells

ASJC Scopus subject areas

  • Transplantation

Cite this

Wehner, J. R., Fox-Talbot, K., Halushka, M. K., Ellis, C., Zachary, A. A., & Baldwin, W. M. (2010). B cells and plasma cells in coronaries of chronically rejected cardiac transplants. Transplantation, 89(9), 1141-1148. https://doi.org/10.1097/TP.0b013e3181d3f271

B cells and plasma cells in coronaries of chronically rejected cardiac transplants. / Wehner, Jennifer R.; Fox-Talbot, Karen; Halushka, Marc K; Ellis, Carla; Zachary, Andrea A.; Baldwin, William M.

In: Transplantation, Vol. 89, No. 9, 05.2010, p. 1141-1148.

Research output: Contribution to journalArticle

Wehner, JR, Fox-Talbot, K, Halushka, MK, Ellis, C, Zachary, AA & Baldwin, WM 2010, 'B cells and plasma cells in coronaries of chronically rejected cardiac transplants', Transplantation, vol. 89, no. 9, pp. 1141-1148. https://doi.org/10.1097/TP.0b013e3181d3f271
Wehner, Jennifer R. ; Fox-Talbot, Karen ; Halushka, Marc K ; Ellis, Carla ; Zachary, Andrea A. ; Baldwin, William M. / B cells and plasma cells in coronaries of chronically rejected cardiac transplants. In: Transplantation. 2010 ; Vol. 89, No. 9. pp. 1141-1148.
@article{f481fe019f0b4989849c7d682c3b310a,
title = "B cells and plasma cells in coronaries of chronically rejected cardiac transplants",
abstract = "BACKGROUND: Previously, we reported that transcripts of immunoglobulins were increased in coronary arteries dissected from cardiac transplants with arteriopathy, but the prevelance and patterns of B cell and plasma cell infiltration in cardiac allografts has not been documented. METHODS: In this study, we documented the frequency and distribution of B cells and plasma cells in 16 cardiac transplants with advanced chronic rejection that were explanted during a second transplant procedure. Coronary arteries with pathologically confirmed allograft vasculopathy and controls with native atherosclerosis were immunohistologically stained for markers of T cells, B cells, plasma cells, IgG subclasses, C4d, CD21, and CXCL13. RESULTS: We found that B cells and plasma cells were prevalent in most of the samples analyzed (14 of 16) and were distributed in three patterns: adventitial nodules, diffuse adventitial infiltrates, and neointimal infiltrates. These cells were found most frequently in nodules, some of which had distinct compartmentalization and granular C4d deposits on follicular dendritic cells (FDCs) that typify tertiary lymphoid nodules. FDCs also stained for CD21 and CXCL13. Diffuse infiltrates of B cells and plasma cells were found in fibrotic areas of the neointima and adventitia. Only a minority of control coronaries with atherosclerosis contained B cells. CONCLUSIONS: B cells and plasma cell infiltrates are consistent findings in and around coronary arteries with allograft vasculopathy and are significantly more frequent than in coronaries with native atherosclerosis. The presence of C4d on FDCs in tertiary lymphoid nodules suggests active antigen presentation.",
keywords = "B cells, Chronic allograft vasculopathy, Plasma cells",
author = "Wehner, {Jennifer R.} and Karen Fox-Talbot and Halushka, {Marc K} and Carla Ellis and Zachary, {Andrea A.} and Baldwin, {William M.}",
year = "2010",
month = "5",
doi = "10.1097/TP.0b013e3181d3f271",
language = "English (US)",
volume = "89",
pages = "1141--1148",
journal = "Transplantation",
issn = "0041-1337",
publisher = "Lippincott Williams and Wilkins",
number = "9",

}

TY - JOUR

T1 - B cells and plasma cells in coronaries of chronically rejected cardiac transplants

AU - Wehner, Jennifer R.

AU - Fox-Talbot, Karen

AU - Halushka, Marc K

AU - Ellis, Carla

AU - Zachary, Andrea A.

AU - Baldwin, William M.

PY - 2010/5

Y1 - 2010/5

N2 - BACKGROUND: Previously, we reported that transcripts of immunoglobulins were increased in coronary arteries dissected from cardiac transplants with arteriopathy, but the prevelance and patterns of B cell and plasma cell infiltration in cardiac allografts has not been documented. METHODS: In this study, we documented the frequency and distribution of B cells and plasma cells in 16 cardiac transplants with advanced chronic rejection that were explanted during a second transplant procedure. Coronary arteries with pathologically confirmed allograft vasculopathy and controls with native atherosclerosis were immunohistologically stained for markers of T cells, B cells, plasma cells, IgG subclasses, C4d, CD21, and CXCL13. RESULTS: We found that B cells and plasma cells were prevalent in most of the samples analyzed (14 of 16) and were distributed in three patterns: adventitial nodules, diffuse adventitial infiltrates, and neointimal infiltrates. These cells were found most frequently in nodules, some of which had distinct compartmentalization and granular C4d deposits on follicular dendritic cells (FDCs) that typify tertiary lymphoid nodules. FDCs also stained for CD21 and CXCL13. Diffuse infiltrates of B cells and plasma cells were found in fibrotic areas of the neointima and adventitia. Only a minority of control coronaries with atherosclerosis contained B cells. CONCLUSIONS: B cells and plasma cell infiltrates are consistent findings in and around coronary arteries with allograft vasculopathy and are significantly more frequent than in coronaries with native atherosclerosis. The presence of C4d on FDCs in tertiary lymphoid nodules suggests active antigen presentation.

AB - BACKGROUND: Previously, we reported that transcripts of immunoglobulins were increased in coronary arteries dissected from cardiac transplants with arteriopathy, but the prevelance and patterns of B cell and plasma cell infiltration in cardiac allografts has not been documented. METHODS: In this study, we documented the frequency and distribution of B cells and plasma cells in 16 cardiac transplants with advanced chronic rejection that were explanted during a second transplant procedure. Coronary arteries with pathologically confirmed allograft vasculopathy and controls with native atherosclerosis were immunohistologically stained for markers of T cells, B cells, plasma cells, IgG subclasses, C4d, CD21, and CXCL13. RESULTS: We found that B cells and plasma cells were prevalent in most of the samples analyzed (14 of 16) and were distributed in three patterns: adventitial nodules, diffuse adventitial infiltrates, and neointimal infiltrates. These cells were found most frequently in nodules, some of which had distinct compartmentalization and granular C4d deposits on follicular dendritic cells (FDCs) that typify tertiary lymphoid nodules. FDCs also stained for CD21 and CXCL13. Diffuse infiltrates of B cells and plasma cells were found in fibrotic areas of the neointima and adventitia. Only a minority of control coronaries with atherosclerosis contained B cells. CONCLUSIONS: B cells and plasma cell infiltrates are consistent findings in and around coronary arteries with allograft vasculopathy and are significantly more frequent than in coronaries with native atherosclerosis. The presence of C4d on FDCs in tertiary lymphoid nodules suggests active antigen presentation.

KW - B cells

KW - Chronic allograft vasculopathy

KW - Plasma cells

UR - http://www.scopus.com/inward/record.url?scp=77951880037&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77951880037&partnerID=8YFLogxK

U2 - 10.1097/TP.0b013e3181d3f271

DO - 10.1097/TP.0b013e3181d3f271

M3 - Article

C2 - 20386145

AN - SCOPUS:77951880037

VL - 89

SP - 1141

EP - 1148

JO - Transplantation

JF - Transplantation

SN - 0041-1337

IS - 9

ER -