TY - JOUR
T1 - B-cell responses to myelin basic protein and its epitopes in autoimmune encephalomyelitis induced by Semple rabies vaccine
AU - Piyasirisilp, Sucheep
AU - Hemachudha, Thiravat
AU - Griffin, Diane E.
N1 - Funding Information:
This study was supported by `The Representative Benjamin Cardin Grant' from the National Multiple Sclerosis Society.
PY - 1999/8/3
Y1 - 1999/8/3
N2 - Semple rabies vaccine is composed of rabies virus-infected sheep or goat brain inactivated with phenol and is administered daily after exposure for 14-21 days. Semple rabies vaccine-induced autoimmune encephalomyelitis (SAE) has clinico-pathological findings of demyelination similar to experimental autoimmune encephalomyelitis (EAE) caused by injection of central nervous system tissue or purified myelin proteins into experimental animals and frequently studied as a model for the human demyelinating disease, multiple sclerosis (MS). T-cell-mediated immune responses play a major role in induction of EAE, and antibody responses enhance disease severity. We studied the antibody responses to myelin basic protein (MBP) in 24 Thai patients with SAE and 77 control individuals to define the linear epitopes in human MBP that are encephalitogenic. Antibody levels were assessed by ELISA using native human MBP or synthetic MBP peptides of 20 amino acids. The major B-cell epitope was MBP61-80 and a minor epitope was MBP106-140 in SAE while in MS the major B-cell epitope is MBP84-96. MBP61-80-specific IgG1 and IgG3 levels were significantly higher in patients than controls while IgG2 and IgG4 were not. The data support the hypothesis that autoreactive Th1 cells induce SAE. The difference in B-cell epitope recognition may be due to differences in the genetic backgrounds of the populations studied or may reflect underlying differences in the pathogenesis of SAE and MS. Copyright (C) 1999 Elsevier Science B.V.
AB - Semple rabies vaccine is composed of rabies virus-infected sheep or goat brain inactivated with phenol and is administered daily after exposure for 14-21 days. Semple rabies vaccine-induced autoimmune encephalomyelitis (SAE) has clinico-pathological findings of demyelination similar to experimental autoimmune encephalomyelitis (EAE) caused by injection of central nervous system tissue or purified myelin proteins into experimental animals and frequently studied as a model for the human demyelinating disease, multiple sclerosis (MS). T-cell-mediated immune responses play a major role in induction of EAE, and antibody responses enhance disease severity. We studied the antibody responses to myelin basic protein (MBP) in 24 Thai patients with SAE and 77 control individuals to define the linear epitopes in human MBP that are encephalitogenic. Antibody levels were assessed by ELISA using native human MBP or synthetic MBP peptides of 20 amino acids. The major B-cell epitope was MBP61-80 and a minor epitope was MBP106-140 in SAE while in MS the major B-cell epitope is MBP84-96. MBP61-80-specific IgG1 and IgG3 levels were significantly higher in patients than controls while IgG2 and IgG4 were not. The data support the hypothesis that autoreactive Th1 cells induce SAE. The difference in B-cell epitope recognition may be due to differences in the genetic backgrounds of the populations studied or may reflect underlying differences in the pathogenesis of SAE and MS. Copyright (C) 1999 Elsevier Science B.V.
KW - Encephalomyelitis, acute disseminated
KW - Epitopes, B-lymphocyte
KW - Immunoglobulin class switching
KW - Multiple sclerosis
KW - Myelin basic proteins
UR - http://www.scopus.com/inward/record.url?scp=0033052979&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033052979&partnerID=8YFLogxK
U2 - 10.1016/S0165-5728(99)00065-X
DO - 10.1016/S0165-5728(99)00065-X
M3 - Article
C2 - 10430042
AN - SCOPUS:0033052979
SN - 0165-5728
VL - 98
SP - 96
EP - 104
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
IS - 2
ER -