@article{505927ef3d8b4b3b96f6f903aed506a1,
title = "Axonal Motility and Its Modulation by Activity Are Branch-Type Specific in the Intact Adult Cerebellum",
abstract = "We performed two-photon in vivo imaging of cerebellar climbing fibers (CFs; the terminal arbor of olivocerebellar axons) in adult mice. CF ascending branches innervate Purkinje cells while CF transverse branches show a near complete failure to form conventional synapses. Time-lapse imaging over hours or days revealed that ascending branches were very stable. However, transverse branches were highly dynamic, exhibiting rapid elongation and retraction and varicosity turnover. Thus, different branches of the same axon, with different innervation patterns, display branch type-specific motility in the adult cerebellum. Furthermore, dynamic changes in transverse branch length were almost completely suppressed by pharmacological stimulation of olivary firing.",
keywords = "SYSBIO, SYSNEURO",
author = "Hiroshi Nishiyama and Masahiro Fukaya and Masahiko Watanabe and Linden, {David J.}",
note = "Funding Information: We are grateful to Dwight Bergles for insightful advice throughout the project. We also thank Richard Huganir for providing polyclonal GFP antibody, Jeffrey Friedman for providing NPY-tauSapphireGFP BAC transgenic mice, Miguel Sena-Esteves for providing AAV8-CBA-EGFP vector, and members of the Linden and Watanabe laboratories for helpful comments and discussion. This work was supported by NIMH R37 MH51106 to D.J.L., Grant-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Culture, Sports, Science, and Technology, Japan to M.W., and Grant-in-Aid for Young Scientists (B) from Japan Society for the Promotion of Science to M.F. D.J.L. is a member of a Silvio O. Conte Neuroscience Research Center, supported by the NIH. ",
year = "2007",
month = nov,
day = "8",
doi = "10.1016/j.neuron.2007.09.010",
language = "English (US)",
volume = "56",
pages = "472--487",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "3",
}