Axonal Motility and Its Modulation by Activity Are Branch-Type Specific in the Intact Adult Cerebellum

Hiroshi Nishiyama, Masahiro Fukaya, Masahiko Watanabe, David J Linden

Research output: Contribution to journalArticle

Abstract

We performed two-photon in vivo imaging of cerebellar climbing fibers (CFs; the terminal arbor of olivocerebellar axons) in adult mice. CF ascending branches innervate Purkinje cells while CF transverse branches show a near complete failure to form conventional synapses. Time-lapse imaging over hours or days revealed that ascending branches were very stable. However, transverse branches were highly dynamic, exhibiting rapid elongation and retraction and varicosity turnover. Thus, different branches of the same axon, with different innervation patterns, display branch type-specific motility in the adult cerebellum. Furthermore, dynamic changes in transverse branch length were almost completely suppressed by pharmacological stimulation of olivary firing.

Original languageEnglish (US)
Pages (from-to)472-487
Number of pages16
JournalNeuron
Volume56
Issue number3
DOIs
StatePublished - Nov 8 2007

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Cerebellum
Axons
Time-Lapse Imaging
Purkinje Cells
Photons
Synapses
Pharmacology

Keywords

  • SYSBIO
  • SYSNEURO

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Axonal Motility and Its Modulation by Activity Are Branch-Type Specific in the Intact Adult Cerebellum. / Nishiyama, Hiroshi; Fukaya, Masahiro; Watanabe, Masahiko; Linden, David J.

In: Neuron, Vol. 56, No. 3, 08.11.2007, p. 472-487.

Research output: Contribution to journalArticle

Nishiyama, Hiroshi ; Fukaya, Masahiro ; Watanabe, Masahiko ; Linden, David J. / Axonal Motility and Its Modulation by Activity Are Branch-Type Specific in the Intact Adult Cerebellum. In: Neuron. 2007 ; Vol. 56, No. 3. pp. 472-487.
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