TY - JOUR
T1 - Avertin affects murine colitis by regulating neutrophils and macrophages
AU - Wang, Dezhi
AU - Chen, Linxiao
AU - Fu, Yanxia
AU - Kang, Qian
AU - Wang, Xin
AU - Ma, Xianzong
AU - Li, Xuhang
AU - Sheng, Jianqiu
N1 - Funding Information:
This study was supported by Capital's Funds for Health Improvement and Research , Grant No. 2018-1-5091 . Appendix A
Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2020/3
Y1 - 2020/3
N2 - Anesthetics are thought to be involved in immunomodulation. Avertin is one of the safest and most commonly used intravenous anesthetics in rodent experiments; it is also widely used in euthanasia of inflammatory bowel disease (IBD) models. This study aimed to define the role and mechanism of action of Avertin on murine colitis. We assessed the effects of a single Avertin injection on colitis using the disease activity index (DAI), pathology, enzyme-linked immunosorbent assay (ELISA), multiplex-ELISA, flow cytometry, and routine blood examination in wild-type (WT) and dextran sodium sulphate (DSS)-treated mice. Although Avertin caused acute cecitis in WT mice after 24 h and aggravated inflammation in the medium term, it alleviated inflammation in the late stage of DSS-induced colitis according to the DAI. Avertin upregulated MPO production and induced the accumulation of neutrophils and macrophages in intestinal mucosa of both WT and DSS-treated mice; the altered MPO might indicate a change in respiratory burst. However, it exhibited a more effective suppression of inflammatory factors secreted by macrophages as the colitis progressed. Avertin led to an increase in neutrophils and decrease in monocytes in both WT and DSS-treated mice blood. Our findings suggest that Avertin aggravates inflammation in the early and medium terms, but alleviates inflammation in the late stage of colitis by regulating neutrophils and macrophages.
AB - Anesthetics are thought to be involved in immunomodulation. Avertin is one of the safest and most commonly used intravenous anesthetics in rodent experiments; it is also widely used in euthanasia of inflammatory bowel disease (IBD) models. This study aimed to define the role and mechanism of action of Avertin on murine colitis. We assessed the effects of a single Avertin injection on colitis using the disease activity index (DAI), pathology, enzyme-linked immunosorbent assay (ELISA), multiplex-ELISA, flow cytometry, and routine blood examination in wild-type (WT) and dextran sodium sulphate (DSS)-treated mice. Although Avertin caused acute cecitis in WT mice after 24 h and aggravated inflammation in the medium term, it alleviated inflammation in the late stage of DSS-induced colitis according to the DAI. Avertin upregulated MPO production and induced the accumulation of neutrophils and macrophages in intestinal mucosa of both WT and DSS-treated mice; the altered MPO might indicate a change in respiratory burst. However, it exhibited a more effective suppression of inflammatory factors secreted by macrophages as the colitis progressed. Avertin led to an increase in neutrophils and decrease in monocytes in both WT and DSS-treated mice blood. Our findings suggest that Avertin aggravates inflammation in the early and medium terms, but alleviates inflammation in the late stage of colitis by regulating neutrophils and macrophages.
KW - Anesthetic
KW - Avertin
KW - Inflammatory bowel disease
KW - Macrophage
KW - Neutrophil
UR - http://www.scopus.com/inward/record.url?scp=85077659746&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85077659746&partnerID=8YFLogxK
U2 - 10.1016/j.intimp.2019.106153
DO - 10.1016/j.intimp.2019.106153
M3 - Article
C2 - 31931369
AN - SCOPUS:85077659746
SN - 1567-5769
VL - 80
JO - International immunopharmacology
JF - International immunopharmacology
M1 - 106153
ER -