Autoradiographic localization of DI-like dopamine receptors in the forebrain of male and female Japanese quail and their relationship with immunoreactive tyrosine hydroxylase

Gregory F. Ball, Joseph M. Casto, Jacques Balthazart

Research output: Contribution to journalArticle

Abstract

The distribution of Dl-like dopamine receptors was studied in the brain of male and female Japanese quail (Coturniz japonica) by means of quantitative autoradiography with 3H-labelled D1 selective antagonist, SCH 23390, serving as a ligand. A specific, saturable, high affinity binding of this ligand was demonstrated. High densities of binding sites were detected in the lobus parolfactorius, olfactory tubercle, and paleostriatum augmentatum. Medium densities were observed in the entire neostriatum and in the external layers of the optic tectum. Similar levels of binding outlined the paleostriatum primitivum, the nucleus pretectalis and the nucleus intercollicularis. Low but significant levels of receptors were also present in the medial preoptic area at the level of the sexually dimorphic medial preoptic nucleus and throughout the infundibulum, as well as in the ectostriatum, medial and lateral septum, and nucleus accumbens. At the level of the medial septum, just dorsal to the anterior commissure, two circular areas of high receptor density corresponding to the nucleus of the septal commissure were also observed. No sex difference in receptor density could be detected in any of these areas. All areas containing high densities of D1 receptors also contained high densities of tyrosine hydroxylase (TH) fibers. However, certain areas characterized by a high density of TH-immunoreactive fibers did not contain appreciable densities of D1-like dopamine receptors. The distribution of this receptor and its relationship to TH-immunoreactivity is consistent with observations made in other vertebrates, suggesting that the dopaminergic system is evolutionarily highly conserved among amniote vertebrates.

Original languageEnglish (US)
Pages (from-to)121-133
Number of pages13
JournalJournal of Chemical Neuroanatomy
Volume9
Issue number2
DOIs
StatePublished - 1995

Fingerprint

Coturnix
Septal Nuclei
Dopamine Receptors
Tyrosine 3-Monooxygenase
Prosencephalon
Globus Pallidus
Preoptic Area
Vertebrates
Ligands
Neostriatum
Dopamine D1 Receptors
Superior Colliculi
Nucleus Accumbens
Pituitary Gland
Autoradiography
Sex Characteristics
Binding Sites
Brain

Keywords

  • Birds
  • Dopamine receptors
  • Male sexual behavior
  • Preoptic area
  • Quantitative autoradiography

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Autoradiographic localization of DI-like dopamine receptors in the forebrain of male and female Japanese quail and their relationship with immunoreactive tyrosine hydroxylase. / Ball, Gregory F.; Casto, Joseph M.; Balthazart, Jacques.

In: Journal of Chemical Neuroanatomy, Vol. 9, No. 2, 1995, p. 121-133.

Research output: Contribution to journalArticle

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