Autoradiographic localization of calcium channel antagonist receptors in rat brain with [3H]nitrendipine

Robert J. Gould, Kenneth M. Murphy, Snyder Solomon H.

Research output: Contribution to journalArticlepeer-review

Abstract

In vitro autoradiographic techniques have been used to localize [3H]nitrendipine binding sites in the rat brain. The superficial cerebral cortex, the ventral, lateral and posterior nuclei of the thalamus, the molecular layer of the dentate gyrus, the substantia nigra and the external plexiform layer of the olfactory bulb, all contain high densities of silver grains. The level of binding sites are greatly reduced in areas low in synaptic connections. The corpus callosum, the alveus hippocampi and the dorsal commissure of the fornix all lack specific grains, as does the lateral olfactory tract of theolfactory bulb. Specific silver grains are not found in the habenula or the hypothalamus. Grains are not associated with blood vessels profiles. The discrete localizations of [3H]nitrendipine bindings sites suggest a specific synaptic role.

Original languageEnglish (US)
Pages (from-to)217-223
Number of pages7
JournalBrain research
Volume330
Issue number2
DOIs
StatePublished - Mar 25 1985

Keywords

  • [H]nitrendipine
  • autoradiography
  • calcium channel antagonists

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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