Autoradiographic localization of benzomorphan binding sites in rat brain

Barbara J. Crain, Fernando Valdes, Kwen Jen Chang, James O. McNamara

Research output: Contribution to journalArticle

Abstract

The benzomorphan subpopulation of opiate binding sites was labeled by [3H]diprenorphine in the presence of unlabeled ligands selected to quench μ and δ opiate binding sites. The distribution of benzomorphan binding sites was then localized autoradiographically. Areas particularly enriched in these sites were nucleus solitarius, nucleus ambiguus, substantia gelantinosa of the trigeminal nerve, the habenula, and the medial nucleus of the amygdala. Within hippocampal formation, binding was relatively enhanced in the pyramidal and granule cell layers. Within the basal ganglia, binding was greatest in the dorsomedial caudate nucleus and least in the globus pallidus. No 'patches' of increased binding were present in the striatum. The interstitial nucleus of the stria terminalis and the medial preoptic nucleus also showed significant binding. This distribution differs from the distributions of μ, δ and κ opiate binding and is quite similar to the distribution of β-endorphin immunoreactivity. These observations support the hypothesis, based on biochemical studies in brain membranes, that benzomorphan binding sites may represent the ligand recognition sites of putative ε{lunate} receptors.

Original languageEnglish (US)
Pages (from-to)179-186
Number of pages8
JournalEuropean Journal of Pharmacology
Volume113
Issue number2
DOIs
StatePublished - Jul 17 1985
Externally publishedYes

Fingerprint

Benzomorphans
Opiate Alkaloids
Binding Sites
Brain
Diprenorphine
Habenula
Ligands
Endorphins
Mediodorsal Thalamic Nucleus
Septal Nuclei
Medulla Oblongata
Trigeminal Nerve
Solitary Nucleus
Globus Pallidus
Preoptic Area
Pyramidal Cells
Caudate Nucleus
Basal Ganglia
Hippocampus
Membranes

Keywords

  • Benzomorphan binding sites
  • In vitro autoradiography
  • Opiate receptors

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Autoradiographic localization of benzomorphan binding sites in rat brain. / Crain, Barbara J.; Valdes, Fernando; Chang, Kwen Jen; McNamara, James O.

In: European Journal of Pharmacology, Vol. 113, No. 2, 17.07.1985, p. 179-186.

Research output: Contribution to journalArticle

Crain, Barbara J. ; Valdes, Fernando ; Chang, Kwen Jen ; McNamara, James O. / Autoradiographic localization of benzomorphan binding sites in rat brain. In: European Journal of Pharmacology. 1985 ; Vol. 113, No. 2. pp. 179-186.
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