Autoradiographic imaging of phosphoinositide turnover in the brain

Paul M. Hwang, David S. Bredt, Solomon H. Snyder

Research output: Contribution to journalArticlepeer-review

Abstract

With [3H]cytidine as a precursor, phosphoinositide turnover can be localized in brain slices by selective autoradiography of the product [ 3H]cytidine diphosphate diacylglycerol, which is membrane-bound. In the cerebellum, glutamatergic stimulation elicits an increase of phosphoinositide turnover only in Purkinje cells and the molecular layer. In the hippocampus, both glutamatergic and muscarinic cholinergic stimulation increase phosphoinositide turnover, but with distinct localizations. Cholinergic stimulation affects CA1, CA3, CA4, and subiculum, whereas glutamatergic effects are restricted to the subiculum and CA3. Imaging phosphoinositide turnover in brain slices, which are amenable to electrophysiologic studies, will permit a dynamic localized analysis of regulation of this second messenger in response to synaptic stimulation of specific neuronal pathways.

Original languageEnglish (US)
Pages (from-to)802-804
Number of pages3
JournalScience
Volume249
Issue number4970
DOIs
StatePublished - 1990

ASJC Scopus subject areas

  • General

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