Autoradiographic Evidence of [3H]SCH 23390 Binding Site; in Human Prefrontal Cortex (Brodmann's Area 9)

Ted M. Dawson, R. Tyler McCabe, Suzanne S. Stensaas, James K. Wamsley

Research output: Contribution to journalArticlepeer-review

Abstract

Abstract: The distribution of dopamine D‐1 receptors has been determined in human prefrontal cortex (Brodmann's area 9) by an in vitro light microscopic autoradiographic method. Dopamine D‐l receptors were localized by using [3H]SCH 23390 as a ligand. Our results indicated that [3H]‐SCH 23390 binding to slide‐mounted tissue sections of human brain is specific, saturable, and of high affinity. Lamina Va contained the highest density of D‐l receptors, with a Bmax value of 11.2 × 1.3 fmol/mg tissue. The KD values for [3H]SCH 23390 in all laminae ranged from 2.6 to 3.2 nM. Competition studies performed with [3H]SCH 23390 indicated a pharmacologic profile consistent with labeling of the D‐l receptor.

Original languageEnglish (US)
Pages (from-to)789-796
Number of pages8
JournalJournal of Neurochemistry
Volume49
Issue number3
DOIs
StatePublished - Sep 1987
Externally publishedYes

Keywords

  • Autoradiography
  • Brodmann's area 9
  • Dopamine D‐l receptor
  • Human brain
  • Prefrontal cortex
  • [H]SCH 23390

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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