Autoperfused working heart-lung preparation versus hypothermic cardiopulmonary preservation for transplantation.

H. Adachi, C. D. Fraser, G. J. Kontos, A. M. Borkon, G. M. Hutchins, E. Galloway, J. Brawn, B. A. Reitz, W. A. Baumgartner

Research output: Contribution to journalArticlepeer-review

Abstract

The effects of preserving the heart and lungs with an autoperfused working heart-lung preparation or simple hypothermia via cardiopulmonary bypass were studied in 18 dairy calves that had combined heart-lung transplantation. Group 1 (n = 6) served as the control group in which animals were cooled with cardiopulmonary bypass and immediately had allotransplantations. In group 2 (n = 6), cardiopulmonary function was maintained in the autoperfusion circuit for 4 hours, followed by transplantation. In group 3 (n = 6), the organs were harvested after cooling by cardiopulmonary bypass, stored in cold (4 degrees C) saline solution for 4 hours, and then transplanted. Cardiopulmonary function was compared between the three groups for 6 hours after implantation. Cardiac function was determined by the ratio of the end-systolic pressure to end-systolic dimension. Pulmonary function was evaluated by the measurement of extravascular lung water, arterial oxygenation on 100% inspired oxygen static lung compliance, and histologic lung injury score. All measurements in groups 2 and 3 were similar to those of the control group at 6 hours after implantation. One may use either the hypothermic cardiopulmonary preservation method after cardiopulmonary bypass or the autoperfused working heart-lung preparation for distant organ procurement and expect adequate cardiopulmonary function after transplantation.

Original languageEnglish (US)
Pages (from-to)253-260
Number of pages8
JournalThe Journal of heart transplantation
Volume6
Issue number5
StatePublished - 1987

ASJC Scopus subject areas

  • Transplantation

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