Automethylation activities within the mixed lineage leukemia-1 (MLL1) core complex reveal evidence supporting a "two-active site" model for multiple histone H3 lysine 4 methylation

Anamika Patel; Valarie E. Vought; Stephen Swatkoski; Susan Viggiano; Benny Howard; Venkatasubramanian Dharmarajan; Kelsey E. Monteith; Gillian Kupakuwana; Kevin E. Namitz; Stephen A. Shinsky; Robert J. Cotter; Michael S. Cosgrove

doi:10.1074/jbc.M113.501064

Automethylation activities within the mixed lineage leukemia-1 (MLL1) core complex reveal evidence supporting a "two-active site" model for multiple histone H3 lysine 4 methylation

Anamika Patel, Valarie E. Vought, Stephen Swatkoski, Susan Viggiano, Benny Howard, Venkatasubramanian Dharmarajan, Kelsey E. Monteith, Gillian Kupakuwana, Kevin E. Namitz, Stephen A. Shinsky, Robert J. Cotter, Michael S. Cosgrove

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Background: The MLL1 core complex mono-and dimethylates histone H3 lysine 4 (H3K4). Results: MLL1 automethylates a conserved cysteine residue in its active site cleft. Conclusion: MLL1 automethylation is inhibited by unmodified histone H3 but not by histones previously mono-, di-, or trimethylated at H3K4.

Original language	English (US)
Pages (from-to)	868-884
Number of pages	17
Journal	Journal of Biological Chemistry
Volume	289
Issue number	2
DOIs	https://doi.org/10.1074/jbc.M113.501064
State	Published - Jan 10 2014
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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10.1074/jbc.M113.501064

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Patel, A., Vought, V. E., Swatkoski, S., Viggiano, S., Howard, B., Dharmarajan, V., Monteith, K. E., Kupakuwana, G., Namitz, K. E., Shinsky, S. A., Cotter, R. J., & Cosgrove, M. S. (2014). Automethylation activities within the mixed lineage leukemia-1 (MLL1) core complex reveal evidence supporting a "two-active site" model for multiple histone H3 lysine 4 methylation. Journal of Biological Chemistry, 289(2), 868-884. https://doi.org/10.1074/jbc.M113.501064

Automethylation activities within the mixed lineage leukemia-1 (MLL1) core complex reveal evidence supporting a "two-active site" model for multiple histone H3 lysine 4 methylation. / Patel, Anamika; Vought, Valarie E.; Swatkoski, Stephen et al.
In: Journal of Biological Chemistry, Vol. 289, No. 2, 10.01.2014, p. 868-884.

Research output: Contribution to journal › Article › peer-review

Patel, A, Vought, VE, Swatkoski, S, Viggiano, S, Howard, B, Dharmarajan, V, Monteith, KE, Kupakuwana, G, Namitz, KE, Shinsky, SA, Cotter, RJ & Cosgrove, MS 2014, 'Automethylation activities within the mixed lineage leukemia-1 (MLL1) core complex reveal evidence supporting a "two-active site" model for multiple histone H3 lysine 4 methylation', Journal of Biological Chemistry, vol. 289, no. 2, pp. 868-884. https://doi.org/10.1074/jbc.M113.501064

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abstract = "Background: The MLL1 core complex mono-and dimethylates histone H3 lysine 4 (H3K4). Results: MLL1 automethylates a conserved cysteine residue in its active site cleft. Conclusion: MLL1 automethylation is inhibited by unmodified histone H3 but not by histones previously mono-, di-, or trimethylated at H3K4.",

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AU - Viggiano, Susan

AU - Howard, Benny

AU - Dharmarajan, Venkatasubramanian

AU - Monteith, Kelsey E.

AU - Kupakuwana, Gillian

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AU - Shinsky, Stephen A.

AU - Cotter, Robert J.

AU - Cosgrove, Michael S.

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Automethylation activities within the mixed lineage leukemia-1 (MLL1) core complex reveal evidence supporting a "two-active site" model for multiple histone H3 lysine 4 methylation

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