Automatic cell segmentation in fluorescence images of conuent cell monolayers using multi-object geometric deformable model

Zhen Yang, John A. Bogovic, Aaron Carass, Mao Ye, Peter C. Searson, Jerry L. Prince

Research output: Chapter in Book/Report/Conference proceedingConference contribution


With the rapid development of microscopy for cell imaging, there is a strong and growing demand for image analysis software to quantitatively study cell morphology. Automatic cell segmentation is an important step in image analysis. Despite substantial progress, there is still a need to improve the accuracy, efficiency, and adaptability to different cell morphologies. In this paper, we propose a fully automatic method for segmenting cells in uorescence images of conuent cell monolayers. This method addresses several challenges through a combination of ideas. 1) It realizes a fully automatic segmentation process by first detecting the cell nuclei as initial seeds and then using a multi-object geometric deformable model (MGDM) for final segmentation. 2) To deal with different defects in the uorescence images, the cell junctions are enhanced by applying an orderstatistic filter and principal curvature based image operator. 3) The final segmentation using MGDM promotes robust and accurate segmentation results, and guarantees no overlaps and gaps between neighboring cells. The automatic segmentation results are compared with manually delineated cells, and the average Dice coefficient over all distinguishable cells is 0:88.

Original languageEnglish (US)
Title of host publicationMedical Imaging 2013
Subtitle of host publicationImage Processing
StatePublished - 2013
EventMedical Imaging 2013: Image Processing - Lake Buena Vista, FL, United States
Duration: Feb 10 2013Feb 12 2013

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherMedical Imaging 2013: Image Processing
Country/TerritoryUnited States
CityLake Buena Vista, FL


  • Cell junction network
  • Cell nuclei
  • Cell segmentation
  • Immunouorescence microscopy
  • Multi-object geometric deformable model (MGDM)

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging


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