Automated two dimensional microfluidic protein profiling combining IEF and SDS gel electrophoresis

J. S. Buch, J. Liu, S. Yang, C. W. Tsao, T. Song, P. Sivanesan, K. Phalnikar, C. S. Lee, D. L. DeVoe

Research output: Contribution to conferencePaper

Abstract

A fully automated multidimensional protein separation system is presented as a high-throughput alternative to traditional 2-D PAGE for comprehensive protein profiling and biomarker discovery. Here we present recent developments enabling separation of complex protein mixtures using a new chip design, a substantially modified fabrication process including the use of UV-ozone surface treatments, and in situ photopolymerization of poly(ethylene glycol)-based gel plugs to control pressure induced flow in selected regions of the chip.

Original languageEnglish (US)
Pages1579-1581
Number of pages3
StatePublished - Jan 1 2006
Event10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan
Duration: Nov 5 2006Nov 9 2006

Other

Other10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
CountryJapan
CityTokyo
Period11/5/0611/9/06

Keywords

  • 2-D PAGE
  • Gel electrophoresis
  • Isoelectric focusing

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Buch, J. S., Liu, J., Yang, S., Tsao, C. W., Song, T., Sivanesan, P., Phalnikar, K., Lee, C. S., & DeVoe, D. L. (2006). Automated two dimensional microfluidic protein profiling combining IEF and SDS gel electrophoresis. 1579-1581. Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan.