Automated two dimensional microfluidic protein profiling combining IEF and SDS gel electrophoresis

J. S. Buch; J. Liu; S. Yang; C. W. Tsao; T. Song; P. Sivanesan; K. Phalnikar; C. S. Lee; D. L. DeVoe

Automated two dimensional microfluidic protein profiling combining IEF and SDS gel electrophoresis

J. S. Buch, J. Liu, S. Yang, C. W. Tsao, T. Song, P. Sivanesan, K. Phalnikar, C. S. Lee, D. L. DeVoe

Research output: Contribution to conference › Paper › peer-review

Abstract

A fully automated multidimensional protein separation system is presented as a high-throughput alternative to traditional 2-D PAGE for comprehensive protein profiling and biomarker discovery. Here we present recent developments enabling separation of complex protein mixtures using a new chip design, a substantially modified fabrication process including the use of UV-ozone surface treatments, and in situ photopolymerization of poly(ethylene glycol)-based gel plugs to control pressure induced flow in selected regions of the chip.

Original language	English (US)
Pages	1579-1581
Number of pages	3
State	Published - 2006
Externally published	Yes
Event	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan Duration: Nov 5 2006 → Nov 9 2006

Other

Other	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
Country/Territory	Japan
City	Tokyo
Period	11/5/06 → 11/9/06

Keywords

2-D PAGE
Gel electrophoresis
Isoelectric focusing

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

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title = "Automated two dimensional microfluidic protein profiling combining IEF and SDS gel electrophoresis",

abstract = "A fully automated multidimensional protein separation system is presented as a high-throughput alternative to traditional 2-D PAGE for comprehensive protein profiling and biomarker discovery. Here we present recent developments enabling separation of complex protein mixtures using a new chip design, a substantially modified fabrication process including the use of UV-ozone surface treatments, and in situ photopolymerization of poly(ethylene glycol)-based gel plugs to control pressure induced flow in selected regions of the chip.",

keywords = "2-D PAGE, Gel electrophoresis, Isoelectric focusing",

author = "Buch, {J. S.} and J. Liu and S. Yang and Tsao, {C. W.} and T. Song and P. Sivanesan and K. Phalnikar and Lee, {C. S.} and DeVoe, {D. L.}",

year = "2006",

language = "English (US)",

pages = "1579--1581",

note = "10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 ; Conference date: 05-11-2006 Through 09-11-2006",

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TY - CONF

T1 - Automated two dimensional microfluidic protein profiling combining IEF and SDS gel electrophoresis

AU - Buch, J. S.

AU - Liu, J.

AU - Yang, S.

AU - Tsao, C. W.

AU - Song, T.

AU - Sivanesan, P.

AU - Phalnikar, K.

AU - Lee, C. S.

AU - DeVoe, D. L.

PY - 2006

Y1 - 2006

N2 - A fully automated multidimensional protein separation system is presented as a high-throughput alternative to traditional 2-D PAGE for comprehensive protein profiling and biomarker discovery. Here we present recent developments enabling separation of complex protein mixtures using a new chip design, a substantially modified fabrication process including the use of UV-ozone surface treatments, and in situ photopolymerization of poly(ethylene glycol)-based gel plugs to control pressure induced flow in selected regions of the chip.

AB - A fully automated multidimensional protein separation system is presented as a high-throughput alternative to traditional 2-D PAGE for comprehensive protein profiling and biomarker discovery. Here we present recent developments enabling separation of complex protein mixtures using a new chip design, a substantially modified fabrication process including the use of UV-ozone surface treatments, and in situ photopolymerization of poly(ethylene glycol)-based gel plugs to control pressure induced flow in selected regions of the chip.

KW - 2-D PAGE

KW - Gel electrophoresis

KW - Isoelectric focusing

UR - http://www.scopus.com/inward/record.url?scp=84902472827&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84902472827&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:84902472827

SP - 1579

EP - 1581

T2 - 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006

Y2 - 5 November 2006 through 9 November 2006

ER -

Automated two dimensional microfluidic protein profiling combining IEF and SDS gel electrophoresis

Abstract

Other

Keywords

ASJC Scopus subject areas

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Cite this