Automated recognition of patterns characteristic of subcellular structures in fluorescence microscopy images

Michael V. Boland, Mia K. Markey, Robert F. Murphy

Research output: Contribution to journalArticlepeer-review

Abstract

Methods for numerical description and subsequent classification of cellular protein localization patterns are described. Images representing the localization patterns of 4 proteins and DNA were obtained using fluorescence microscopy and divided into distinct training and test sets. The images were processed to remove out-of-focus and background fluorescence and 2 sets of numeric features were generated: Zernike moments and Haralick texture features. These feature sets were used as inputs to either a classification tree or a neural network. Classifier performance (the average percent of each type of image correctly classified) on previously unseen images ranged from 63% for a classification tree using Zernike moments to 88% for a backpropagation neural network using a combination of features from the 2 feature sets. These results demonstrate the feasibility of applying pattern recognition methods to subcellular localization patterns, enabling sets of previously unseen images from a single class to be classified with an expected accuracy greater than 99%. This will provide not only a new automated way to describe proteins, based on localization rather than sequence, but also has potential application in the automation of microscope functions and in the field of gene discovery.

Original languageEnglish (US)
Pages (from-to)366-375
Number of pages10
JournalCytometry
Volume33
Issue number3
DOIs
StatePublished - Nov 1 1998
Externally publishedYes

Keywords

  • Fluorescence
  • Microscopy
  • Neural networks (computer)
  • Pattern recognition
  • Protein localization
  • Subcellular location
  • Zernike moments

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Biophysics
  • Hematology
  • Endocrinology
  • Cell Biology

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