Automated grouping of action potentials of human embryonic stem cell-derived cardiomyocytes

Giann Gorospe, Renjun Zhu, Michal A. Millrod, Elias T. Zambidis, Leslie Tung, Rene Vidal

Research output: Contribution to journalArticle

Abstract

Methods for obtaining cardiomyocytes from human embryonic stem cells (hESCs) are improving at a significant rate. However, the characterization of these cardiomyocytes (CMs) is evolving at a relatively slower rate. In particular, there is still uncertainty in classifying the phenotype (ventricular-like, atrial-like, nodal-like, etc.) of an hESC-derived cardiomyocyte (hESC-CM). While previous studies identified the phenotype of a CM based on electrophysiological features of its action potential, the criteria for classification were typically subjective and differed across studies. In this paper, we use techniques from signal processing and machine learning to develop an automated approach to discriminate the electrophysiological differences between hESC-CMs. Specifically, we propose a spectral grouping-based algorithm to separate a population of CMs into distinct groups based on the similarity of their action potential shapes. We applied this method to a dataset of optical maps of cardiac cell clusters dissected from human embryoid bodies. While some of the nine cell clusters in the dataset are presented with just one phenotype, the majority of the cell clusters are presented with multiple phenotypes. The proposed algorithm is generally applicable to other action potential datasets and could prove useful in investigating the purification of specific types of CMs from an electrophysiological perspective.

Original languageEnglish (US)
Article number6766211
Pages (from-to)2389-2395
Number of pages7
JournalIEEE Transactions on Biomedical Engineering
Volume61
Issue number9
DOIs
StatePublished - Sep 2014

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Keywords

  • Cardiac electrophysiology
  • cardiomyocyte (CM)
  • spectral grouping
  • stem cells.

ASJC Scopus subject areas

  • Biomedical Engineering

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