Automated DNA sizing by atomic force microscopy

Thomas S. Spisz, Ye Fang, Isaac N. Bankman, Roger H. Reeves, Jan H. Hoh

Research output: Contribution to journalArticlepeer-review

Abstract

DNA sizing is one of the most widely used analytical methods in molecular biology and biochemistry, and it is a core method for genomic analysis. Many research, health care, and forensic applications rely on DNA sizing. Given the volume of DNA sizing done and the associated cost, an increasing need exists for extremely rapid, high-throughput, and inexpensive techniques. Essentially all DNA sizing today is performed by gel electrophoresis, although multiple efforts are under way to develop more effective technologies based on entirely new approaches. One such effort is described in this article: the use of atomic force microscopy in combination with pattern recognition software to perform DNA sizing. Initial results show that this approach can be compared with gel electrophoresis for some applications. Future work aims at developing a practical, high-throughput DNA sizing system.

Original languageEnglish (US)
Pages (from-to)135-142
Number of pages8
JournalJohns Hopkins APL Technical Digest (Applied Physics Laboratory)
Volume20
Issue number2
StatePublished - Jan 1 1999

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

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